Mutants of Saccharomyces cerevisiae with defective vacuolar function

Journal Article · Wed Jun 01 00:00:00 EDT 1988 · J. Bacteriol.; (United States)

OSTI ID:6765380

Kitamoto, K; Yoshizawa, K; Ohsumi, Y; Anraku, Y

Mutants of the yeast Saccharomyces cerevisiae that have a small vacuolar lysine pool were isolated and characterized. Mutant KL97 (lys1 slp1-1) and strain KL197-1A (slp1-1), a prototrophic derivative of KL97, did not grow well in synthetic medium supplemented with 10 mM lysine. Genetic studies indicated that the slp1-1mutation (for small lysine pool) is recessive and is due to a single chromosomal mutation. Mutant KL97 shows the following pleiotropic defects in vacuolar functions. (i) It has small vacuolar pools for lysine, arginine, and histidine. (ii) Its growth is sensitive to lysine, histidine, Ca/sup 2 +/, heavy metal ions, and antibiotics. (iii) It has many small vesicles but no large central vacuole. (iv) It has a normal amount of the vacuolar membrane marker ..cap alpha..-mannosidase but shows reduced activities of the vacuole sap markers proteinase A, proteinase B, and carboxypeptidase Y.

• Research Organization: National Research Institute of Brewing, Tokyo (Japan)
• OSTI ID: 6765380
• Journal Information: J. Bacteriol.; (United States), Vol. 170:6
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTIBIOTICS
BIOLOGICAL EFFECTS
CALCIUM COMPOUNDS
METALS
SACCHAROMYCES CEREVISIAE
GENE MUTATIONS
METABOLISM
BIOLOGICAL FUNCTIONS
HISTIDINE
LYSINE
MUTANTS
SENSITIVITY
ALKALINE EARTH METAL COMPOUNDS
AMINO ACIDS
ANTI-INFECTIVE AGENTS
AZOLES
CARBOXYLIC ACIDS
DRUGS
ELEMENTS
FUNCTIONS
FUNGI
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
MICROORGANISMS
MUTATIONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
SACCHAROMYCES
YEASTS
560300* - Chemicals Metabolism & Toxicology