Alkaline peroxide delignification of agricultural residues to enhance enzymatic saccharification. [Trichoderma reesei]
Approximately one-half of the lignin and most of the hemicellulose present in agricultural residues such as wheat straw and corn stover are solubilized when the residue is treated at 25/sup 0/C in an alkaline solution of hydrogen peroxide. The delignification reaction is most efficient when the ratio of hydrogen peroxide to substrate is at least 0.25 (w/w) and the pH is 11.5. The supernatant fraction from a given pretreatment, after addition of makeup peroxide and readjustment of the pH, can be recycled to treat at least six additional batches of substrate, resulting in a substantial concentration of hemicellulose and soluble lignin degradation products. Hydrolysis of the insoluble fraction with Trichoderma reesei cellulase after alkline peroxide treatment yields glucose with almost 100% efficiency, based upon the cellulose content of the residue before treatment. These data indicate that alkaline peroxide pretreatment is a simple and efficient method for enhancing the enzymatic digestibility of lignocellulosic crop residues to levels approaching the theoretical maximum.
- Research Organization:
- Dept. of Agriculture, Peoria, IL
- OSTI ID:
- 6762970
- Journal Information:
- Biotechnol. Bioeng.; (United States), Vol. 26:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AGRICULTURAL WASTES
DELIGNIFICATION
CELLULOSE
BIOCONVERSION
ETHANOL
BIOSYNTHESIS
GLUCOSE
HEMICELLULOSE
ENZYMATIC HYDROLYSIS
LIGNIN
AUGMENTATION
CELLULOLYTIC ACTIVITY
CHEMICAL REACTIONS
HYDROGEN PEROXIDE
ALCOHOLS
ALDEHYDES
CARBOHYDRATES
DECOMPOSITION
ENZYME ACTIVITY
HEXOSES
HYDROGEN COMPOUNDS
HYDROLYSIS
HYDROXY COMPOUNDS
LYSIS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC WASTES
OXYGEN COMPOUNDS
PEROXIDES
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
SYNTHESIS
WASTES
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)