Purification and immobilization of Aspergillus niger. beta. -xylosidase

Journal Article · Tue Jan 01 00:00:00 EST 1980 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260220603· OSTI ID:6758804

Oguntimein, G B; Reilly, P J

..beta..-Xylosidase from a commercial Aspergillus niger preparation was purified by differential ammonium sulfate precipitation and either gel permeation or cation exchange chromatography, giving 16-fold purification in 32% yield for the first technique or 27-fold purification in 19% yield for the second. Enzyme prepared by this method was immobilized to 10 different carriers, but only when it was bound to alumina with TiCl/sub 4/ and to alkylamine porous silica with glutaraldehyde were substantial efficiencies and stabilities achieved.

• Research Organization: Iowa State Univ., Ames
• OSTI ID: 6758804
• Journal Information: Biotechnol. Bioeng.; (United States), Vol. 22
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
09 BIOMASS FUELS
ALUMINIUM OXIDES
CARRIER MOBILITY
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PRECIPITATION
PURIFICATION
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ALUMINIUM COMPOUNDS
CHALCOGENIDES
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