Luminal hydrolysis of recombinant human epidermal growth factor in the rat gastrointestinal tract: segmental and developmental differences

Journal Article · Fri Jan 01 00:00:00 EST 1988 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(88)90299-8· OSTI ID:6691412

Britton, J R; George-Nascimento, C; Koldovsky, O

Epidermal growth factor (EGF), present in high concentrations in the milk of various species, is biologically active following oral administration to young animals. To characterize the luminal metabolism of dietary EGF in the developing gastrointestinal tract, the authors incubated human recombinant /sup 125/I-EGF in vitro at 37/sup 0/ with luminal fluid from the stomach and various segments of the small intestine of 12 day old suckling and 31 day old weanling rats and analyzed the resulting reaction products. The rate of EGF hydrolysis as determined by generation of acid soluble material was greater in weanling small intestine than in suckling, with maximal hydrolytic capacity observed in the mid-jejunum and ilium. Minimal hydrolysis was observed with stomach fluid from both age groups, and EGF retained its ability to elute as a single species on Sephadex G-25 columns and to bind to monospecific affinity columns and placental membrane receptors. Incubation with suckling small intestinal fluid produced little change in the chromatographic profile on Sephadex G-25, but a reduction in antibody and receptor binding was observed. In contrast, incubation with weanling small intestinal fluid yielded both a more pronounced loss of EGF-like material on G-25 columns and a greater reduction in receptor and antibody binding.

• Research Organization: Arizona Health Sciences Center, Tucson (USA)
• OSTI ID: 6691412
• Journal Information: Life Sci.; (United States), Vol. 43:17
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GROWTH FACTORS
METABOLISM
AGE DEPENDENCE
CHROMATOGRAPHY
DIET
EPIDERMIS
GASTROINTESTINAL TRACT
HYDROLYSIS
IODINE 125
MAN
MILK
RATS
TRACER TECHNIQUES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
EPITHELIUM
FOOD
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LYSIS
MAMMALS
MATERIALS
MITOGENS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RADIOISOTOPES
RODENTS
SEPARATION PROCESSES
SKIN
SOLVOLYSIS
TISSUES
VERTEBRATES
550501* - Metabolism- Tracer Techniques