Effect of maturation on gastrointestinal absorption of epidermal growth factor in rats

Journal Article · Wed Jul 01 04:00:00 EDT 1987 · Am. J. Physiol.; (United States)

OSTI ID:6145868

Thornburg, W; Rao, R K; Matrisian, L M; Magun, B E; Koldovsky, O

Epidermal growth factor (EGF) was iodinated and administered orally to 13- to 15-day-old suckling rats and 29- to 31-day-old weanling rats. After 30 min, stomach, small intestine, plasma, liver, lung, and skin were removed. The tissues were homogenized and /sup 125/I radioactivity was extracted. Compared with suckling rats, the delivery of total radioactivity into peripheral tissues was enhanced in skin of weanling rats and tended to be higher in plasma and liver. In contrast, there was a 3.3-fold reduction in radioactivity remaining in the intestinal wall. Sephadex G-25 chromatography of most samples, especially liver and intestinal wall, revealed a decrease in the proportion of intact /sup 125/I-EGF eluting in the void volume. As a result, because the amount of total radioactivity also differed, the overall recovery of radioactivity of void volume /sup 125/I-EGF was similar in both age groups except for an increase in skin and a decrease in the intestinal of weanling rats. Extracts of all tissues of weanling rats examined contained immunoreactive /sup 125/I-EGF. Samples obtained from tissues and content of the gastrointestinal tract of both age groups bound specifically to A431 cell surface receptors. These results thus indicate that EGF is absorbed and delivered to various tissues of weanling rats. Nevertheless, quantitative and qualitative changes in these processes occur during the postnatal period.

• Research Organization: Univ. of Arizona College of Medicine, Tucson (USA)
• OSTI ID: 6145868
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 253:1; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
FOOD
GASTROINTESTINAL TRACT
GLANDS
GROWTH FACTORS
HALOGENATION
INTERMEDIATE MASS NUCLEI
INTESTINAL ABSORPTION
INTESTINES
IODINATION
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
LIVER
LUNGS
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MILK
MITOGENS
NUCLEI
ODD-EVEN NUCLEI
ORAL ADMINISTRATION
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
RATS
RECEPTORS
RESPIRATORY SYSTEM
RODENTS
SEPARATION PROCESSES
SKIN
SMALL INTESTINE
STOMACH
TISSUE DISTRIBUTION
UPTAKE
VERTEBRATES