Inhibition of gamma-ray dose-rate effects by D/sup 2/O and inhibitors of poly(ADP-ribose) synthetase in cultured mammalian L5178Y cells

Journal Article · Fri Jun 01 00:00:00 EDT 1984 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576489· OSTI ID:6668321

Ueno, A M; Tanaka, O; Matsudaira, H

Effects of deuterium oxide (D/sub 2/O) and 3-aminobenzamide, an inhibitor of poly(ADP-ribose) synthetase, on cell proliferation and survival were studied in cultured mammalian L5178Y cells under growing conditions and after acute and low-dose-rate irradiation at about 0.1 to 0.4 Gy/hr of ..gamma.. rays. Growth of irradiated and unirradiated cells was inhibited by 45% D/sub 2/O but not by 3-aminobenzamide at 10mM, except for treatments longer than 30 hr. The presence of these agents either alone or in combination during irradiation at low dose rates suppressed almost totally the decrease in cell killing due to the decrease in dose rate. Among other inhibitors tested, theobromine and theophylline were found to be effective in eliminating the dose-rate effects of ..gamma.. rays. Possible mechanisms underlying the inhibition are discussed.

• Research Organization: National Inst. of Radiological Sciences, Chiba, Japan
• OSTI ID: 6668321
• Journal Information: Radiat. Res.; (United States), Vol. 98:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CELL KILLING
RADIOINDUCTION
ENZYME INHIBITORS
BIOLOGICAL EFFECTS
TUMOR CELLS
BIOLOGICAL RADIATION EFFECTS
CELL PROLIFERATION
DOSE RATES
EXPERIMENTAL DATA
GAMMA RADIATION
HEAVY WATER
LIGASES
MICE
SURVIVAL CURVES
ANIMAL CELLS
ANIMALS
DATA
ELECTROMAGNETIC RADIATION
ENZYMES
HYDROGEN COMPOUNDS
INFORMATION
IONIZING RADIATIONS
MAMMALS
NUMERICAL DATA
OXYGEN COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RODENTS
VERTEBRATES
WATER
560121* - Radiation Effects on Cells- External Source- (-1987)