Inhibition of potentially lethal radiation damage repair in normal and neoplastic human cells by 3-aminobenzamide: an inhibitor of poly(ADP-ribosylation)

Journal Article · Fri Aug 01 00:00:00 EDT 1986 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(86)90212-9· OSTI ID:7023341

Thraves, P J; Mossman, K L; Frazier, D T; Dritschilo, A

The effect of 3-aminobenzamide (3AB), an inhibitor of poly(ADP-ribose) synthetase, on potentially lethal damage repair (PLDR) was investigated in normal human fibroblasts and four human tumor cell lines from tumors with varying degrees of radiocurability. The tumor lines selected were: Ewing's sarcoma, a bone tumor considered radiocurable and, human lung adenocarcinoma, osteosarcoma, and melanoma, three tumors considered nonradiocurable. PLDR was measured by comparing cell survival when cells were irradiated in a density-inhibited state and replated at appropriate cell numbers at specified times following irradiation to cell survival when cells were replated immediately following irradiation. 3AB was added to cultures 2 hr prior to irradiation and removed at the time of replating. Different test radiation doses were used for the various cell lines to obtain equivalent levels of cell survival. In the absence of inhibitor, PLDR was similar in all cell lines tested. In the presence of 8 mM 3AB, differential inhibition of PLDR was observed. PLDR was almost completely inhibited in Ewing's sarcoma cells and partially inhibited in normal fibroblast cells and osteosarcoma cells. No inhibition of PLDR was observed in the lung adenocarcinoma or melanoma cells. Except for the osteosarcoma cells, inhibition of PLDR by 3AB correlated well with radiocurability.

• Research Organization: Georgetown Univ. Medical Center, Washington, DC
• OSTI ID: 7023341
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Vol. 8
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
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RADIOSENSITIVITY EFFECTS
OSTEOSARCOMAS
RADIOSENSITIVITY
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL REPAIR
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ORGANIC NITROGEN COMPOUNDS
RADIATION EFFECTS
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RECOVERY
REPAIR
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SKELETAL DISEASES
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560120* - Radiation Effects on Biochemicals
Cells
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