Electrophoretic and electro-optical studies on the conformation and susceptibility to psoralen crosslinking of magnetically oriented DNA

Technical Report · Sun Aug 01 00:00:00 EDT 1982

OSTI ID:6642467

Roots, R J; Kraft, G H; Farinato, R S; Tenforde, T S

Gel electrophoresis and electro-optical birefringence measurements were performed on the replicative form of bacteriophage phi X-174 DNA subjected to orientation in a homogeneous stationary magnetic field. The conformation of this superhelical double-stranded form of DNA, and its sensitivity to intercalation and crosslinking by a psoralen derivative, were found to be unaffected by a 1 h exposure to a 2.15 Tesla field. In addition, no alteration was detected in the infectivity of the exposed phi X-174 DNA in E. coli bacterial hosts.

• Research Organization: Lawrence Berkeley Lab., CA (USA)
• DOE Contract Number: AC03-76SF00098
• OSTI ID: 6642467
• Report Number(s): LBL-13601; ON: DE83004045
• Resource Relation: Other Information: Portions of document are illegible. Printed copy available until stock is exhausted
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIOPHAGES
INFECTIVITY
DNA
CONFORMATIONAL CHANGES
MAGNETIC FIELDS
BIOLOGICAL EFFECTS
BIREFRINGENCE
ELECTROPHORESIS
ORIENTATION
PSORALEN
SENSITIVITY
ANTICOAGULANTS
COUMARINS
DRUGS
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PARASITES
REFRACTION
VIRUSES
560400* - Other Environmental Pollutant Effects