Photochemistry of psoralen-DNA adducts, biological effects of psoralen-DNA adducts, applications of psoralen-DNA photochemistry
This thesis consists of three main parts and totally eight chapters. In Part I, The author will present studies on the photochemistry of psoralen-DNA adducts, specifically, the wavelength dependencies for the photoreversals of thymidine-HMT (4'-hydroxymethyl-4, 5', 8-trimenthylpsoralen) monoadducts and diadduct and the same adducts incorporated in DNA helices and the wavelength dependecies for the photocrossslinking of thymidine-HMT monoadducts in double-stranded helices. In Part II, The author will report some biological effects of psoralen-DNA adducts, i.e., the effects on double-stranded DNA stability, DNA structure, and transcription by E. coli and T7 RNA polymerases. Finally, The author will focus on the applications of psoralen-DNA photochemistry to investigation of protein-DNA interaction during transcription, which includes the interaction of E. coli and T7 RNA polymerases with DNA in elongation complexes arrested at specific psoralen-DNA adduct sites as revealed by DNase I footprinting experiments. 123 refs., 52 figs., 12 tabs.
- Research Organization:
- Lawrence Berkeley Lab., CA (USA)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 5069947
- Report Number(s):
- LBL-25057; ON: DE88010879
- Country of Publication:
- United States
- Language:
- English
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ADDUCTS
ANTICOAGULANTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHEMICAL REACTION KINETICS
COUMARINS
DAYS LIVING RADIOISOTOPES
DNA ADDUCTS
DRUGS
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
ENZYMES
FREQUENCY DEPENDENCE
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
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LABELLED COMPOUNDS
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NUCLEI
NUCLEIC ACIDS
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PHOSPHORUS 32
PHOSPHORUS ISOTOPES
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POLYMERASES
PSORALEN
RADIATIONS
RADIOISOTOPES
REACTION KINETICS
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RNA
TRACER TECHNIQUES
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TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION