Stabilization of k-carrageenan gel with polymeric amines: use of immobilized cells as biocatalysts at elevated temperatures

Journal Article · Mon Sep 01 00:00:00 EDT 1986 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260280902· OSTI ID:6629317

Chao, K C; Haugen, M M; Royer, G P

Spherical beads of kappa-carrageenan containing entrapped cells were prepared in a two-step process. First, the beads were formed by dispersing a warm carrageenan cell suspension into stirring oil. After cooling (gelation) the beads were cured by treatment with amines. Ten amines of various sizes and structures were tested. The mechanical strength and the applicability of amine-treated gels as immobilization matrices was evaluated. The results of critical compression tests indicate that linear and branched polyethylenimines (PEI) are both good curing agents. PEI-treated carrageenan beds also exhibited superior resistance to heat and abrasion. Furthermore, PEI polymers were demonstrated to be effective in stabilizing the lactase activity of the free and immobilized Bacillus stearothermophilus cells. The immobilized cell preparations of Saccharomyces cerevisiae, B. stearothermophilus, and Flavobacterium sp. were treated with branched PEI and evaluated for the activity of invertase (EC 3.2.1.26), lactase (EC 3.2.1.23), and glucose isomerase (EC 5.3.1.18), respectively, in a packed bed reactor at 60 degrees C. The apparent half-lives were 108, 39, and 64 days, respectively.

• Research Organization: Amoco Corp., Napeville, IL
• OSTI ID: 6629317
• Journal Information: Biotechnol. Bioeng.; (United States), Vol. 28:9
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
CATALYST SUPPORTS
CHEMICAL PREPARATION
AMINES
BACILLUS
BIOREACTORS
COMPRESSION
CULTURE MEDIA
ENZYME ACTIVITY
GELS
IMMOBILIZED CELLS
PACKED BED
SACCHAROMYCES CEREVISIAE
BACTERIA
COLLOIDS
DISPERSIONS
FUNGI
MICROORGANISMS
ORGANIC COMPOUNDS
PLANTS
SACCHAROMYCES
SYNTHESIS
YEASTS
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