Flow cytometric determination of radiation-induced chromosome damage and its correlation with cell survival
Chinese hamster M3-1 cells were irradiated with several doses of x rays or ..cap alpha.. particles from /sup 238/Pu. Propidium iodide-stained chromosome suspensions were prepared at different times after irradiation; cells were also assayed for survival. The DNA histograms of these chromosomes showed increased background counts with increased doses of radiation. This increase in background was cell-cycle dependent and was correlated with cell survival. The correlation between radiation-induced chromosome damage and cell survival was the same for X rays and ..cap alpha.. particles. Data are presented which indicate that flow cytometric analysis of chromosomes of irradiated cell populations can be a useful adjunct to classical cytogenic analysis of irradiation-induced chromosomal damage by virtue of its ability to express and measure chromosomal damage not seen by classical cytogenic methods.
- Research Organization:
- Los Alamos National Lab., New Mexico
- OSTI ID:
- 6597979
- Journal Information:
- Radiat. Res.; (United States), Vol. 99:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CELL KILLING
RADIOINDUCTION
CHROMOSOMAL ABERRATIONS
FIBROBLASTS
BIOLOGICAL RADIATION EFFECTS
ALPHA PARTICLES
CAFFEINE
CELL CYCLE
CELL FLOW SYSTEMS
HAMSTERS
PLUTONIUM 238
SURVIVAL CURVES
X RADIATION
ACTINIDE ISOTOPES
ACTINIDE NUCLEI
ALPHA DECAY RADIOISOTOPES
ANALEPTICS
ANIMAL CELLS
ANIMALS
AROMATICS
AZAARENES
BIOLOGICAL EFFECTS
CENTRAL NERVOUS SYSTEM AGENTS
CHARGED PARTICLES
CONNECTIVE TISSUE CELLS
DRUGS
ELECTROMAGNETIC RADIATION
EVEN-EVEN NUCLEI
HEAVY NUCLEI
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
ISOTOPES
MAMMALS
MUTATIONS
NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PLUTONIUM ISOTOPES
PURINES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
VERTEBRATES
XANTHINES
YEARS LIVING RADIOISOTOPES
560121* - Radiation Effects on Cells- External Source- (-1987)
550400 - Genetics