Induction of chromosome damage by ultraviolet light and caffeine: correlation of cytogenetic evaluation and flow karyotype

Journal Article · Fri Jan 01 00:00:00 EST 1982 · Cytometry (Baltimore); (United States)

DOI:https://doi.org/10.1002/cyto.990020504· OSTI ID:6842454

Cremer, C; Cremer, T; Gray, J W

Asynchronously growing cells of a M3-1 Chinese hamster line were ultraviolet (uv) irradiated (lambda = 254 nm) with uv fluences up to 7.5 J/m/sup 2/. After irradiation, cells were incubated with or without 2 mM caffeine for 20 hr, then mitotic cells were selected by mechanical shaking. Their chromosomes were isolated, stained with Hoechst 33258 and chromomycin A3, and measured flow cytometrically. While the fluorescence distributions of chromosomes (flow karyotypes) from cells treated with uv alone or with caffeine alone were very similar to those of untreated controls, the flow karyotypes of uv + caffeine-treated cells showed a debris continuum that increased with increasing uv fluence suggesting an increased number of chromosome fragments. Visual evaluation of metaphase plates revealed that the percentage of cells with chromosome damage also increased steadily with increasing uv fluence. A high degree of correlation was observed between the relative magnitude of the debris level from flow karyotypes and the percentage of cells with chromosome damage and with generalized chromosome shattering, respectively, as determined from metaphase spreads.

• Research Organization: Lawrence Livermore National Lab., CA
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 6842454
• Journal Information: Cytometry (Baltimore); (United States), Vol. 2:5
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CAFFEINE
RADIOSENSITIVITY EFFECTS
CHROMOSOMES
BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
CELL FLOW SYSTEMS
CHROMOSOMAL ABERRATIONS
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KARYOTYPE
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RADIATION DOSES
ULTRAVIOLET RADIATION
ANALEPTICS
ANIMALS
BIOLOGICAL EFFECTS
CELL DIVISION
CENTRAL NERVOUS SYSTEM AGENTS
DOSES
DRUGS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
MAMMALS
MUTATIONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PURINES
RADIATION EFFECTS
RADIATIONS
RODENTS
VERTEBRATES
XANTHINES
560111* - Radiation Effects on Biochemicals- In Vitro- (-1987)
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