Slit-scanning analysis of dicentric chromosomes: Different approaches for an automated biological dosimetry

Conference · Fri Jan 01 00:00:00 EST 1993 · Cytometry (Baltimore); (United States)

OSTI ID:6558537

Beisker, W; Weller, E M; Nuesse, M ^[1]

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1. GSF-Inst. fuer Biophysikalische Strahlenforschung, Neuherberg (Germany)

The analysis of dicentric chromosomes is an important method for applications in biological dosimetry. Automation using slit-scanning flow cytometric analysis requires a careful estimation of preparational and instrumental artifacts. As long as reliable techniques for FISH techniques or immunological kinetochore staining in suspension have not been developed, special interest is directed to methods for evaluating artifacts by instrumental or mathematical procedures. The authors present applications for detection of dicentric chromosomes in mammalian cell cultures as well as in human lymphocytes by multiparameter slit-scanning techniques. The improvement of the sensitivity by mathematical analysis of the profiles as well as by simultaneous use of different DNA dyes with multilaser fluorescence excitation is discussed.

• OSTI ID: 6558537
• Report Number(s): CONF-9303114-; CODEN: CYTODQ
• Journal Information: Cytometry (Baltimore); (United States), Vol. 6; Conference: 16. congress of the International Society for Analytical Cytology, Colorado Springs, CO (United States), 21-26 Mar 1993; ISSN 0196-4763
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CYTOLOGICAL TECHNIQUES
AUTOMATION
DICENTRIC CHROMOSOMES
DETECTION
CELL CULTURES
DOSIMETRY
FLUORESCENCE
HYBRIDIZATION
LASERS
LYMPHOCYTES
MAMMALS
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMOSOMES
CONNECTIVE TISSUE CELLS
LEUKOCYTES
LUMINESCENCE
MATERIALS
SOMATIC CELLS
VERTEBRATES
550300* - Cytology
550400 - Genetics