Detection and Automated Scoring of Dicentric Chromosomes in Nonstimulated Lymphocyte Prematurely Condensed Chromosomes After Telomere and Centromere Staining

Journal Article · Sun Mar 01 04:00:00 EST 2015 · International Journal of Radiation Oncology, Biology and Physics

DOI:https://doi.org/10.1016/J.IJROBP.2014.10.048· OSTI ID:22458635

M'kacher, Radhia ^[1]; El Maalouf, Elie ^[1]; Terzoudi, Georgia ^[2]; Ricoul, Michelle ^[1]; Heidingsfelder, Leonhard ^[3]; Karachristou, Ionna ^[2]; Laplagne, Eric ^[4]; Hempel, William M. ^[1]; Colicchio, Bruno; Dieterlen, Alain ^[5]; Pantelias, Gabriel ^[2]; Sabatier, Laure ^[1]

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1. Laboratoire de Radiobiologie et Oncologie, Commissariat à l'Energie Atomique, Fontenay-aux-Roses (France)
2. Laboratory of Radiobiology & Biodosimetry, National Center for Scientific Research Demokritos, Athens (Greece)
3. MetaSystems, Altlussheim (Germany)
4. Pole Concept, Paris (France)
5. Laboratoire Modélisation Intelligence Processus Systèmes (MIPS)–Groupe TIIM3D, Université de Haute-Alsace, Mulhouse (France)

Purpose: To combine telomere and centromere (TC) staining of premature chromosome condensation (PCC) fusions to identify dicentrics, centric rings, and acentric chromosomes, making possible the realization of a dose–response curve and automation of the process. Methods and Materials: Blood samples from healthy donors were exposed to {sup 60}Co irradiation at varying doses up to 8 Gy, followed by a repair period of 8 hours. Premature chromosome condensation fusions were carried out, and TC staining using peptide nucleic acid probes was performed. Chromosomal aberration (CA) scoring was carried out manually and automatically using PCC-TCScore software, developed in our laboratory. Results: We successfully optimized the hybridization conditions and image capture parameters, to increase the sensitivity and effectiveness of CA scoring. Dicentrics, centric rings, and acentric chromosomes were rapidly and accurately detected, leading to a linear-quadratic dose–response curve by manual scoring at up to 8 Gy. Using PCC-TCScore software for automatic scoring, we were able to detect 95% of dicentrics and centric rings. Conclusion: The introduction of TC staining to the PCC fusion technique has made possible the rapid scoring of unstable CAs, including dicentrics, with a level of accuracy and ease not previously possible. This new approach can be used for biological dosimetry in radiation emergency medicine, where the rapid and accurate detection of dicentrics is a high priority using automated scoring. Because there is no culture time, this new approach can also be used for the follow-up of patients treated by genotoxic therapy, creating the possibility to perform the estimation of induced chromosomal aberrations immediately after the blood draw.

• OSTI ID: 22458635
• Journal Information: International Journal of Radiation Oncology, Biology and Physics, Journal Name: International Journal of Radiation Oncology, Biology and Physics Journal Issue: 3 Vol. 91; ISSN IOBPD3; ISSN 0360-3016
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
AUTOMATION
BIOLOGICAL REPAIR
CENTROMERES
CHROMOSOMAL ABERRATIONS
COBALT 60
DIAGRAMS
DICENTRIC CHROMOSOMES
DOSIMETRY
DRUGS
HYBRIDIZATION
IRRADIATION
LYMPHOCYTES
NUCLEIC ACIDS
PATIENTS
PEPTIDES
RADIATION DOSES
SENSITIVITY
STAINS
THERAPY