Increased incorporation of /sup 14/C-palmitate into tissue lipids by isolated heart myocytes in endotoxic shock
The incorporation of /sup 14/C-palmitate into various classes of tissue lipids by isolated adult dog heart myocytes was studied in an attempt to understand the pathophysiology of myocardial dysfunction during endotoxic shock. The results showed that the incorporation of /sup 14/C-palmitate into phospholipids was increased by 85.3% and 108.8% at 0.5 hours and two hours, respectively, following endotoxin (0.5 mg Escherichia coli lipopolysaccharide B per kg body weight) administration. Incorporation of radioactive palmitate into triglycerides was increased by 50.9% and 107.2% at 0.5 and two hours, respectively, postendotoxin. Incorporation of /sup 14/C-palmitate into diglycerides was stimulated by 51.9% and 64.5% at 0.5 and two hours, respectively, after endotoxin injection. The incorporation of /sup 14/C-palmitate into tissue-free fatty acids and unaltered at 0.5 hours but it was increased by 211.7% at two hours postendotoxin. These data demonstrated that myocardial membrane lipid profile was greatly altered by increased incorporation of /sup 14/C-palmitate into phospholipids and neutral lipids after endotoxin administration. An alteration in myocardial lipid profile, as reported in this study, may contribute to the development of myocardial dysfunction during shock.
- OSTI ID:
- 6530690
- Journal Information:
- Adv. Shock Res.; (United States), Vol. 7
- Country of Publication:
- United States
- Language:
- English
Similar Records
Triacylglycerol kinetics in endotoxic rats with suppressed lipoprotein lipase activity
Effects of substrate availability on myocardial C-11 palmitate kinetics by positron emission tomography in normal subjects and patients with ventricular dysfunction
Related Subjects
CARBON 14 COMPOUNDS
TRACER TECHNIQUES
CARDIOVASCULAR DISEASES
PATHOLOGY
BIOLOGICAL SHOCK
DOGS
ENDOTOXINS
HEART
HEXADECANOIC ACID
LIPIDS
PHYSIOLOGY
ANIMALS
ANTIGENS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
DISEASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
TOXIC MATERIALS
TOXINS
VERTEBRATES
550901* - Pathology- Tracer Techniques
551001 - Physiological Systems- Tracer Techniques