Human T-cell leukemia virus types I and II exhibit different DNase I protection patterns

Journal Article · Fri Apr 01 00:00:00 EST 1988 · Journal of Virology; (USA)

OSTI ID:6528929

Altman, R; Harrich, D; Garcia, J A ^[1]; Gaynor, R B ^[2]

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1. Univ. of California Los Angeles School of Medicine (USA)
2. Univ. of California Los Angeles School of Medicine (USA) Wadsworth Veterans Hospital, Los Angeles, CA (USA)

Human T-cell leukemia virus types I (HTLV-I) and II (HTLV-II) are human retroviruses which normally infect T-lymphoid cells. HTLV-I infection is associated with adult T-cell leukemia-lymphoma, and HTLV-II is associated with an indolent form of hairy-cell leukemia. To identify potential transcriptional regulatory elements of these two related human retroviruses, the authors performed DNase I footprinting of both the HTLV-I and HTLV-II long terminal repeats (LTRs) by using extracts prepared from uninfected T cells, HTLV-I and HTLV-II transformed T cells, and HeLa cells. Five regions of the HTLV-I LTR and three regions of the HTLV-II LTR showed protection by DNase I footprinting. All three of the 21-base-pair repeats previously shown to be important in HTLV transcriptional regulation were protected in the HTLV-I LTR, whereas only one of these repeats was protected in the HTLV-II LTR. Several regions exhibited altered protection in extracts prepared from lymphoid cells as compared with HeLa cells, but there were minimal differences in the protection patterns between HTLV-infected and uninfected lymphoid extracts. A number of HTLV-I and HTLV-II LTR fragments which contained regions showing protection in DNase I footprinting were able to function as inducible enhancer elements in transient CAT gene expression assays in the presence of the HTLV-II tat protein. The alterations in the pattern of the cellular proteins which bind to the HTLV-I and HTLV-II LTRs may in part be responsible for differences in the transcriptional regulation of these two related viruses.

• OSTI ID: 6528929
• Journal Information: Journal of Virology; (USA), Vol. 62:4; ISSN 0022-538X
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENE OPERONS
DNA HYBRIDIZATION
GENE REPRESSORS
LEUKEMIA VIRUSES
MOLECULAR BIOLOGY
ONCOGENIC TRANSFORMATIONS
DNA-ASE
HELA CELLS
LYMPHOCYTES
MAN
PHOSPHORUS 32
RFLPS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL TRANSFORMATIONS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ENZYMES
ESTERASES
HYBRIDIZATION
HYDROLASES
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
MAMMALS
MATERIALS
MICROORGANISMS
NUCLEI
NUCLEOPROTEINS
ODD-ODD NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHOSPHODIESTERASES
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
SOMATIC CELLS
VERTEBRATES
VIRUSES
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