Characterization of the human granulocyte-macrophage colony-stimulating factor promoter region by genetic analysis: Correlation with DNase I footprinting

Journal Article · Sun May 01 04:00:00 EDT 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.5.1979· OSTI ID:6995312

Nimer, S D; Morita, E A; Martis, M J; Wachsman, W; Gasson, J C

T-cell activation induces expression of the hematopoietic growth factor granulocyte-macrophage colony-stimulating factor (GM-CSF). To define the molecular events involved in the induction of GM-CSF gene expression more clearly, the authors prepared and analyzed deletion mutants of GM-CSF promoter recombinant constructs. The results localized inducible expression to a 90-base-pair region (-53 to +37) which is active in uninfected and human T-cell leukemia virus-infected T-cell lines but not in resting or mitogen-stimulated B cells. DNase I footprinting experiments revealed protection of sequences contained within this region, including a repeated nucleotide sequence, CATT(A/T), which could serve as a core recognition sequence for a cellular transcription factor. Upstream of these GM-CSF promoter sequences is a 15-base-pair region (-193 to -179) which has negative regulatory activity in human T-cell leukemia virus-infected T cells. These studies revealed a complex pattern of regulation of GM-CSF expression in T cells; positive and negative regulatory sequences may play critical roles in controlling the expression of this potent granulopoietin in the bone marrow microenvironment and in localized inflammatory responses.

• Research Organization: Div. of Hematology-Oncology, UCLA School of Medicine and Jonsson Comprehensive Cancer Center, Los Angeles, CA (US)
• OSTI ID: 6995312
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:5; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DATA
DNA
DNA SEQUENCING
ENZYME INDUCTION
EXPERIMENTAL DATA
GENE OPERONS
GENE REGULATION
GENETIC MAPPING
GROWTH FACTORS
INFORMATION
LEUKOCYTES
LYMPHOCYTES
MACROPHAGES
MAMMALS
MAN
MAPPING
MATERIALS
MITOGENS
MUTANTS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
PHAGOCYTES
PHENOTYPE
PRIMATES
PROTEINS
RECOMBINANT DNA
SOMATIC CELLS
STIMULATION
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
VERTEBRATES