Immediate-early gene region of human cytomegalovirus trans-activates the promoter of human immunodeficiency virus

Journal Article · Tue Dec 01 00:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.23.8642· OSTI ID:6468174

Davis, M G; Kenney, S C; Kamine, J; Pagano, J S; Huang, E S

Almost all homosexual patients with acquired immunodeficiency syndrome are also actively infected with human cytomegalovirus (HCMV). The authors have hypothesized that an interaction between HCMV and human immunodeficiency virus (HIV), the agent that causes acquired immunodeficiency syndrome, may exist at a molecular level and contribute to the manifestations of HIV infection. In this report, they demonstrate that the immediate-early gene region of HCMV, in particular immediate-early region 2, trans-activates the expression of the bacterial gene chloramphenicol acetyltransferase that is fused to the HIV long terminal repeat and carried by plasmid pHIV-CAT. The HCMV immediate-early trans-activator increases the level of mRNA from the plamid pHIV-CAT. The sequences of HIV that are responsive to trans-activation by the HDMV immediate-early region are distinct from HIV sequences that are required for response to the HIV tat. The stimulation of HIV gene expression by HDMV gene functions could enhance the consequences of HIV infection in persons with previous or concurrent HCMV infection.

• Research Organization: Univ. of North Carolina, Chapel Hill (USA)
• OSTI ID: 6468174
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:23
• Country of Publication: United States
• Language: English

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