Attachment and proliferation of bovine aortic endothelial cells onto additive modified poly(ether urethane ureas)

Journal Article · Thu Apr 01 00:00:00 EST 1993 · Journal of Biomedical Materials Research; (United States)

DOI:https://doi.org/10.1002/jbm.820270409· OSTI ID:6466313

Brunstedt, M R; Ziats, N P; Rose-Caprara, V; Hiltner, P A; Anderson, J M ^[1]; Lodoen, G A; Payet, C R ^[2]

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1. Case Western Reserve Univ., Cleveland, OH (United States)
2. E.I. du Pont de Nemours and Co., Waynesboro, VA (United States) E.I. du Pont de Nemours and Co., Wilmington, DE (United States)

To better understand endothelial cell interactions with poly(ether urethane urea) (PEUU) materials, and to assess bovine aortic endothelial cell attachment, films were incubated for 24 h with BAEC in media containing 5% fetal bovine serum. Other films were allowed to incubate for 4 more days in media containing 5% fetal bovine serum without cells to assess BAEC proliferation. The assay was performed on PEUU films modified with acrylate and methacrylate polymer and copolymer additives that spanned a wide range on the hydrophobicity/hydrophilicity scale. Tissue culture polystyrene (TCPS) was used as a control. The assay showed that PEUU films loaded with Methacrol 2138F [copoly(diisopropylaminoethyl methacrylate [DIPAM]/decyl methacrylate [DM]) (3/1)] or with its hydrophilic component, DIPAM, in homopolymer form (i.e., h-DIPAM), significantly enhanced BAEC attachment and proliferation when compared to unloaded PEUU films or to PEUU films loaded with the more hydrophobic acrylate or methacrylate polymer additives. The assay also showed that PEUU films coated with homopoly(diisopropylaminoethyl acrylate) (h-DIPAA) significantly enhanced BAEC attachment and proliferation when compared to PEUU films coated with h-decyl acrylate films coated with the copolymer of these two acrylates showed intermediate behavior.

• OSTI ID: 6466313
• Journal Information: Journal of Biomedical Materials Research; (United States), Vol. 27:4; ISSN 0021-9304
• Country of Publication: United States
• Language: English

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