Characterization and regulation of the gene coding for human glutathione-insulin transhydrogenase (protein-disulfide interchange enzyme)
Glutathione-insulin transhydrogenase (GIT) is an ubiquitious enzyme whose action is to catalyze the rearrangement of disulfide bonds in proteins, resulting in either the activation or inactivation of protein molecules. The interaction of GIT with insulin results in the cleavage of the hormone into its constituent subunits, the A and B chains. GIT levels found in several tissue types have been shown to be modulated by circulating levels of insulin. An increased level of blood insulin is associated with increased levels of GIT and decreased levels of insulin are associated with decreased levels of GIT, indicating that insulin regulates its own degradation. A cDNA probe coding for the GIT gene was isolated from a human liver cDNA expression library constructed in lambda gt11. Sequencing of this clone indicates that it codes for the 3' half of the gene through the poly-A tail region. There is high homology between this cDNA to human GIT and a published sequence for rat liver cDNA. The clone was used as a probe for quantitation and characterization of GIT mRNA in rat tissues by use of RNA Dot blots and Northern blots. Only one species of mRNA having 2300 bases was found in tissues screened thus far (liver, spleen, kidney, and testis). This mRNA is sufficiently large to code for a protein the size of GIT (58,000 d). Work is in progress to compare mRNA levels as influenced by insulin levels.
- Research Organization:
- Wright State Univ., Dayton, OH
- OSTI ID:
- 6417288
- Report Number(s):
- CONF-870644-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GENES
DNA SEQUENCING
HYDROGENASES
BIOCHEMICAL REACTION KINETICS
GENE REGULATION
INSULIN
BIOSYNTHESIS
CLEAVAGE
BLOOD
KIDNEYS
LIVER
MAN
MESSENGER-RNA
RATS
SPLEEN
TESTES
ANIMALS
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CRYSTAL STRUCTURE
DIGESTIVE SYSTEM
ENZYMES
GLANDS
GONADS
HORMONES
KINETICS
MALE GENITALS
MAMMALS
MATERIALS
MICROSTRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEPTIDE HORMONES
PRIMATES
REACTION KINETICS
RNA
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
VERTEBRATES
550200* - Biochemistry