Clofibrate-induced cytochrome P450-lauric acid omega hydroxylase(P450LA omega):purification, cDNA cloning, sequence and regulation
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:7171579
A cytochrome P450 that hydroxylates lauric acid at the 12 position (P450LA omega) was isolated from liver microsomes of clofibrate treated rats. P450LA omega was immunologically distinct from P450s a,b,c,d,e,f,g,h,j,PB1, and PCN1. Polyclonal antibody against P450LA omega was utilized to screen a gt11 cDNA library. A clone (pP450LA omega), was isolated and its sequence determined. The P450LA omega mRNA is a minimum 2387 nts in length and codes for a P450 of Mr.58,222 daltons. This protein shares less than 35% amino acid similarity with P450s b,c,d,e,f,PB1, and PCN1; however, it does contain a hydrophobic amino terminal peptide and a conserved sequence surrounding the Cys residue at position 456, which is similar to other microsomal P450s. P450LA omega is present at high levels in untreated rat kidney and is induced by clofibrate in both kidney and liver. This induction is the result of an accumulation of mRNA through a rapid transcriptional activation of the P450LA gene. Southern blotting data suggest the presence of 2 or 3 genes in the P450LA omega family. This P450 gene family may be associated with arachidonic acid and prostraglandin metabolism in kidney and other tissues.
- Research Organization:
- Argonne National Labs., IL
- OSTI ID:
- 7171579
- Report Number(s):
- CONF-8606151-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
BODY
CELL CONSTITUENTS
CLONING
CYTOCHROMES
DIGESTIVE SYSTEM
DNA SEQUENCING
DNA-CLONING
ENZYMES
GENES
GLANDS
HYDROXYLASES
KIDNEYS
LIVER
MAMMALS
MESSENGER-RNA
MICROSOMES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
PIGMENTS
PROTEINS
PURIFICATION
RATS
RNA
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
BODY
CELL CONSTITUENTS
CLONING
CYTOCHROMES
DIGESTIVE SYSTEM
DNA SEQUENCING
DNA-CLONING
ENZYMES
GENES
GLANDS
HYDROXYLASES
KIDNEYS
LIVER
MAMMALS
MESSENGER-RNA
MICROSOMES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
PIGMENTS
PROTEINS
PURIFICATION
RATS
RNA
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
VERTEBRATES