Sources of error in screening by cytometry for the effects of environmental mutagens

Journal Article · Fri Jan 01 00:00:00 EST 1993 · Environmental Toxicology and Chemistry; (United States)

DOI:https://doi.org/10.1002/etc.5620120106· OSTI ID:6382370

Tiersch, T R ^[1]; Wachtel, S S ^[2]

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1. Memphis State Univ., TN (United States)
2. Univ. of Tennessee, Memphis (United States)

Flow cytometry is often used to detect DNA aneuploidy and mosaicism associated with malignancy or genetic damage. Yet DNA aneuploidy and mosaicism detected by flow cytometry may be more apparent than real. In contrast to the DNA mass observed for blood, the authors consistently found markedly different values and higher variability for DNA mass among other tissues collected from the same animal. Prepared mixtures of blood and other cells generated multiple fluorescence peaks identical to those that might be expected for aneuploid mosaicism. Moreover, analysis of tissues such as feather pulp, which contains a combination of cell types, yielded multiple fluorescence peaks that were not observed when blood alone was analyzed. Thus care should be exercised in classifying DNA values from different tissues as normal or abnormal, because the appearance of supernumerary fluorescence peaks might not always indicate the presence of abnormal cell populations.

• OSTI ID: 6382370
• Journal Information: Environmental Toxicology and Chemistry; (United States), Vol. 12:1; ISSN 0730-7268
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
MUTAGEN SCREENING
DATA COVARIANCES
POLLUTANTS
ANIMAL TISSUES
BLOOD
CELL FLOW SYSTEMS
DNA
FLUORESCENCE
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
LUMINESCENCE
MATERIALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
SCREENING
TISSUES
560300* - Chemicals Metabolism & Toxicology