Effects of recombinant eel growth hormone on the uptake of ( sup 35 S)sulfate by ceratobranchial cartilages of the Japanese eel, Anguilla japonica
- Univ. of Tokyo (Japan)
Effects of growth hormone (GH) on the synthesis of mucopolysaccharide by ceratobranchial cartilages of the Japanese eel, Anguilla japonica, were examined by monitoring the in vitro uptake of ({sup 35}S)sulfate. The ({sup 35}S)sulfate uptake decreased rapidly to one-third of the initial level during the first 3 days after hypophysectomy, and decreased gradually thereafter. When hypophysectomized eels were injected intramuscularly with recombinant eel GH (2 micrograms/g), the plasma GH concentrations increased maximally after 6 hr, and declined rapidly thereafter. On the other hand, the sulfate uptake increased significantly after 12 hr, and high levels were maintained until 48 hr. The stimulating effect of GH was dose dependent (0.02-2 micrograms/g). However, the addition of eel GH (0.05-5 micrograms/ml) to the culture medium did not affect the sulfate uptake by hypophysectomized eel cartilages, suggesting that the stimulative action of GH on the sulfate uptake by the cartilages is indirect.
- OSTI ID:
- 6370132
- Journal Information:
- General and Comparative Endocrinology; (USA), Vol. 79:2; ISSN 0016-6480
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
POLYSACCHARIDES
BIOSYNTHESIS
STH
BIOCHEMICAL REACTION KINETICS
CARTILAGE
DOSE-RESPONSE RELATIONSHIPS
EEL
SULFATES
SULFUR 35
TRACER TECHNIQUES
ANIMAL TISSUES
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CONNECTIVE TISSUE
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
FISHES
HORMONES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOISOTOPES
REACTION KINETICS
SACCHARIDES
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques