In vivo and in vitro effects of growth hormone on the incorporation of ( UC)leucine into protein of liver and muscle of the eel

Journal Article · Thu Aug 01 04:00:00 EDT 1985 · Gen. Comp. Endocrinol.; (United States)

DOI:https://doi.org/10.1016/0016-6480(85)90381-8· OSTI ID:6181823

Inui, Y; Ishioka, H

In vivo administration of ovine GH (2 micrograms/g body wt) increased ( UC)leucine incorporation into protein of the liver, skeletal muscle, and opercular muscle of hypophysectomized eels. Addition of ovine GH into the medium slightly increased ( UC)leucine incorporation into protein of liver slices during 5 hr in vitro incubation, but did not affect protein synthesis from ( UC)leucine in opercular muscle. In vivo pretreatment with ovine GH 48 hr prior to tissue preparation clearly increased ( UC)leucine incorporation into protein of liver slices in vitro. However, no statistically significant change was observed for in vitro incorporation of ( UC)leucine into protein of opercular muscle of hypophysectomized eels which had been previously treated with ovine GH. These results indicate that ovine GH has a protein anabolic action in the liver and muscle of the eel and that compared to mammals a rather long lag period is needed to elicit such protein anabolic actions of GH in these animals.

• Research Organization: National Research Institute of Aquaculture, Mie, Japan
• OSTI ID: 6181823
• Journal Information: Gen. Comp. Endocrinol.; (United States), Journal Name: Gen. Comp. Endocrinol.; (United States) Vol. 2; ISSN GCENA
• Country of Publication: United States
• Language: English

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550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
DIGESTIVE SYSTEM
EEL
FISHES
GLANDS
HORMONES
HYPOPHYSECTOMY
IN VITRO
LABELLED COMPOUNDS
LEUCINE
LIVER
MEDICINE
MUSCLES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
STH
SURGERY
SYNTHESIS
VERTEBRATES