Insulin-like growth factor II (IGF II) in human brain: regional distribution of IGF II and of higher molecular mass forms

Journal Article · Mon Apr 01 00:00:00 EST 1985 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.82.7.2153· OSTI ID:6300267

Haselbacher, G K; Schwab, M E; Pasi, A; Humbel, R E

Twenty-four distinct areas of human brain were analyzed for the presence of insulin-like growth factor (IGF). As reported for cerebrospinal fluid, only IGF II-like immunoreactivity, but no significant amounts of IGF I-like immunoreactivity, could be found. Upon gel permeation chromatography, two to five distinct size classes were separated on the basis of their immunoreactivity. Radioimmunoassays and a bioassay also gave results indistinguishable from those of serum IGF II. The highest amounts of IGF II-like immunoreactivity occur in the anterior pituitary. This is up to 100 times more than in most other brain regions analyzed. The higher molecular mass immunoreactive species were partially characterized. After immunoaffinity purification, the 38- and 26-kDa species are active in a bioassay. Specific IGF-binding protein activity could be shown after purification of the 38- and 26-kDa species on an IGF-affinity column. The 13-kDa species released significant amounts of 7.5-kDa material. The results are interpreted as evidence for the presence of IGF II synthesized locally in human brain.

• Research Organization: Universitaet Zuerich, Switzerland
• OSTI ID: 6300267
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 82:7
• Country of Publication: United States
• Language: English

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