skip to main content

Title: Role of T cells in sex differences in syngeneic bone marrow transfers

Transferred marrow cells will proliferate in normal mice not exposed to irradiation or any other type of stem cell depletion when five consecutive transfers of 40 million cells are given. Approximately 25% of the mitotic cells are of male donor origin observed cytogenetically in all of the female recipient spleens and marrow analyzed from two weeks to one and one-half years after transfusions. Male donor stem cells are accepted and form a stable component of the self-renewing stem cell pool. In contrast, only 5% female cells are found in male recipients. This sex difference in engraftment is not hormonal since castration of recipients does not alter the percentage of donor cells. Rigorous T depletion of female donor bone marrow, however, increases the percentage of donor engraftment to the level observed when male marrow, either whole or T depleted, is transferred to female recipients. The success of T-depleted female stem cells to seed male recipients is observed in both C57BL/6 and CBA/J. In addition, recipient nude BALB/c males, which lack a thymus, fail to accept whole bone marrow from BALB/c females. However, male bone marrow cells seed BALB/c nude females. These studies demonstrate that the poor engraftment of female cells inmore » transfused male recipients is abrogated by the removal of T cells from the donor female marrow.« less
Authors:
; ; ;
Publication Date:
OSTI Identifier:
6254626
Resource Type:
Journal Article
Resource Relation:
Journal Name: Exp. Hematol.; (United States); Journal Volume: 10
Research Org:
National Institute of Arthritis, Bethesda, MD
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; BONE MARROW CELLS; TRANSPLANTS; IMMUNITY; SEX DEPENDENCE; IMMUNOSUPPRESSION; RADIOINDUCTION; BONE MARROW; CELL DIVISION; CELL PROLIFERATION; LYMPHOCYTES; MICE; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CONNECTIVE TISSUE CELLS; HEMATOPOIETIC SYSTEM; LEUKOCYTES; MAMMALS; MATERIALS; ORGANS; RODENTS; SOMATIC CELLS; TISSUES; VERTEBRATES 560152* -- Radiation Effects on Animals-- Animals; 550301 -- Cytology-- Tracer Techniques