Daily cortisol production rate in man determined by stable isotope dilution/mass spectrometry
- National Institute of Child Health and Human Development, Bethesda, MD (USA)
Growth retardation as well as the development of Cushingoid features in adrenally insufficient patients treated with the currently accepted replacement dose of cortisol (33-41 mumol/day.m2; 12-15 mg/m2.day) prompted us to reevaluate the cortisol production rate (FPR) in normal subjects and patients with Cushing's syndrome, using a recently developed thermospray liquid chromatography-mass spectrometry method. The stable isotope (9,12,12-2H3)cortisol was infused continuously for 31 h at about 5% of the anticipated FPR. Blood samples were obtained at 20-min intervals for 24 h, spun, and pooled in 4-h groups. Tracer dilution in plasma was determined by liquid chromatography/mass spectrometry. The method was validated with controlled infusions in 6 patients with adrenal insufficiency. Results from 12 normal volunteers revealed a FPR of 27.3 +/- 7.5 mumol/day (9.9 +/- 2.7 mg/day) or 15.7 mumol/day.m2; 5.7 mg/m2. day. A previously unreported circadian variation in FPR was observed. Patients with Cushing's syndrome demonstrated unequivocal elevation of FPR and cortisol concentration correlated during each sample period in normal volunteers, indicating that cortisol secretion, rather than metabolism, is mainly responsible for changes in plasma cortisol. Our data suggest that the FPR in normal subjects may be lower than previously believed.
- OSTI ID:
- 6250177
- Journal Information:
- Journal of Clinical Endocrinology and Metabolism; (USA), Vol. 72:1; ISSN 0021-972X
- Country of Publication:
- United States
- Language:
- English
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ENDOCRINE DISEASES
PATHOGENESIS
HYDROCORTISONE
BIOSYNTHESIS
ADRENAL GLANDS
DEUTERIUM
ISOTOPE DILUTION
LIQUID COLUMN CHROMATOGRAPHY
MAN
MASS SPECTROSCOPY
PATIENTS
ADRENAL HORMONES
ANIMALS
BODY
CHROMATOGRAPHY
CORTICOSTEROIDS
DISEASES
ENDOCRINE GLANDS
GLANDS
GLUCOCORTICOIDS
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
LIGHT NUCLEI
MAMMALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PREGNANES
PRIMATES
SEPARATION PROCESSES
SPECTROSCOPY
STABLE ISOTOPES
STEROIDS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques