Functional atrial natriuretic peptide receptor in human adrenal tumor

Journal Article · Sat Dec 31 23:00:00 EST 1988 · J. Cardiovasc. Pharmacol.; (United States)

DOI:https://doi.org/10.1097/00005344-198905006-00004· OSTI ID:5603524

Shionoiri, H; Hirawa, N; Takasaki, I; Ishikawa, Y; Oda, H; Minamisawa, K; Sugimoto, K; Matsukawa, T; Ueda, S; Miyajima, E

The effects of synthetic human atrial natriuretic peptide (ANP) on the release of catecholamines, aldosterone, or cortisol were observed in human adrenal tumors obtained surgically from patients with pheochromocytoma, primary aldosteronism, or Cushing's syndrome, respectively. Each tumor tissue or adjacent normal cortical tissue was sectioned into slices, which were incubated in medium-199 in the presence or absence of adrenocorticotrophin (ACTH) and ANP. The amounts of epinephrine, norepinephrine, aldosterone, or cortisol released into the medium were measured. Existence of ANP receptors on the adrenal tissues was examined by binding assays, affinity labeling, and immunohistochemistry. Release of catecholamines from pheochromocytoma tissues was inhibited by ANP, and the presence of the ANP receptor on pheochromocytoma was further demonstrated by both binding assays and affinity labeling; Scatchard analysis revealed a single class of binding sites for ANP with a Kd of 1.0 nM and a Bmax of 0.4 pmol/mg of protein and the molecular size was estimated as 140 and a 70 kDa under nonreducing and reducing conditions, respectively. The presence of ANP receptors in pheochromocytoma was demonstrated by immunohistochemistry. ANP inhibited both basal and ACTH-stimulated aldosterone secretion in the slices of normal cortex, and localization of ANP receptors in zona glomerulosa cells was also demonstrated. However, ANP did not inhibit basal and ACTH-stimulated aldosterone and cortisol secretion in both tissue slices from aldosteronoma and Cushing's adenoma. Consistent with these observations, the absence of ANP receptors in adenoma tissues was determined by binding assays, affinity labeling, and immunohistochemistry.

• Research Organization: Yokohama City Univ., School of Medicine (Japan)
• OSTI ID: 5603524
• Journal Information: J. Cardiovasc. Pharmacol.; (United States), Journal Name: J. Cardiovasc. Pharmacol.; (United States) Vol. 13 Suppl 6; ISSN JCPCD
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ADRENAL GLANDS
ADRENAL HORMONES
ADRENALINE
ALDEHYDES
ALDOSTERONE
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BODY
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CELL MEMBRANES
CORTICOSTEROIDS
DISEASES
DRUGS
ENDOCRINE GLANDS
GLANDS
HYDROXY COMPOUNDS
IN VITRO
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
KINETICS
LABELLING
MAMMALS
MAN
MEMBRANE PROTEINS
MEMBRANES
MINERALOCORTICOIDS
NEOPLASMS
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PREGNANES
PRIMATES
PROTEINS
REACTION KINETICS
RECEPTORS
STEROIDS
SYMPATHOMIMETICS
TRACER TECHNIQUES
VERTEBRATES