Increased radiosensitivity of a subpopulation of T-lymphocyte progenitors from patients with Fanconi's anemia
In vitro radiation survival of peripheral blood T lymphocytes was studied in 15 clinically normal adults and 4 patients with Fanconi's anemia. Tritiated thymidine incorporation in a whole blood lymphocyte stimulation test (LST) and a newly developed whole blood T-lymphocyte colony assay were used to measure lymphocyte blastogenesis and colony formation in response to phytohemagglutinin (PHA) or concanavalin-A (Con-A) stimulation. Lymphocyte colony formation was found to be consistently more sensitive than the LST for detection of low-level radiation effects using both normal cells and lymphocytes from Fanconi's anemia patients. Lymphocytes from patients with Fanconi's anemia were significantly more sensitive to in vitro x irradiation than lymphocytes from clinically normal individuals as measured by their ability to divide when stimulated by PHA in the LST and colony formation assay. No significant difference in the radiosensitivity of the Con-A response was observed between the two groups. The PHA-responsive T-lymphocyte subpopulation in Fanconi's anemia patients appears to be intrinsically defective. The nature of this defect, significance in the disease process, and relevancy of these findings to the establishment of radiation protection standards are discussed.
- Research Organization:
- Univ. of California, Davis
- OSTI ID:
- 6232066
- Journal Information:
- Blood; (United States), Vol. 57:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANEMIAS
RADIOSENSITIVITY EFFECTS
LYMPHOCYTES
RADIOSENSITIVITY
COLONY FORMATION
IN VITRO
LOW DOSE IRRADIATION
MITOGENS
PATIENTS
PHYTOHEMAGGLUTININ
STIMULATION
X RADIATION
AGGLUTININS
ANIMAL CELLS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
HEMAGGLUTININS
HEMIC DISEASES
IONIZING RADIATIONS
IRRADIATION
LEUKOCYTES
MATERIALS
MUCOPROTEINS
ORGANIC COMPOUNDS
POLYSACCHARIDES
PROTEINS
RADIATIONS
SACCHARIDES
SOMATIC CELLS
SYMPTOMS
560151* - Radiation Effects on Animals- Man