Growth of human T lymphocyte colonies from whole blood: culture requirements and applications

Journal Article · Fri Jan 01 04:00:00 EST 1982 · J. Cell. Biochem.; (United States)

DOI:https://doi.org/10.1002/jcb.1982.240180103· OSTI ID:6758901

Knox, S J; Wilson, F D; Greenberg, B R; Shifrine, M

Growth of human lymphocyte colonies from whole blood following stimulation with PHA, Con A, or PPD is described. Individual colony cells were identified as T lymphocytes on the basis of surface marker and enzyme cytochemical characterizations. Colony formation increased as a power function over a wide range of cell concentrations above a critical minimal concentration. The whole blood culture system eliminates possible selective effects of lymphocyte colony techniques utilizing gradient-enriched lymphocyte fractions and more closely approximates the in vivo milieu. The whole blood colony method is more sensitive for the detection of low-level radiation effects on lymphocytes than widely used tests that measure /sup 3/H-thymidine incorporation. In preliminary studies, researchers used the whole blood method to determine the relative radiosensitivity of lymphocytes from humans with various hematopoietic disorders, and observed abnormalities in mitogen responsiveness and colony formation in some of the patient groups. This method has wide application for studies in cellular and clinical immunology.

• Research Organization: Laboratory for Energy-Related Health Research, University of California, Davis
• OSTI ID: 6758901
• Journal Information: J. Cell. Biochem.; (United States), Journal Name: J. Cell. Biochem.; (United States) Vol. 18:1; ISSN JCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550301 -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOASSAY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CULTURES
CFU
CLONE CELLS
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DISEASES
ENZYME ACTIVITY
GROWTH
HEMIC DISEASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
QUANTITY RATIO
RADIOSENSITIVITY
SOMATIC CELLS
SURVIVAL CURVES
TRACER TECHNIQUES
TRITIUM COMPOUNDS