Cytometry of deoxyribonuclei acid content and morphology of mammalian sperm

Journal Article · Sat Jan 01 00:00:00 EST 1983 · J. Dairy Sci.; (United States)

DOI:https://doi.org/10.3168/jds.S0022-0302(83)82136-5· OSTI ID:6204257

Gledhill, B L

Because spermatogenesis is exquisitely sensitive to external influences, sperm can serve as a biological dosimeter. Advances in interpreting induced sperm abnormalities require a better understanding of sperm characteristics. This report reviews the application of several methods for automated, quantitative detection of shape changes, methods that are faster and more sensitive than conventional subjective technqiues. Variability of sperm deoxyribonucleic acid content as a bioassay of genetic damage is explored, and limitations of the bioassay are discussed. New flow cytometric techniques that could lead to sexing mammalian sperm are examined.

• Research Organization: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 6204257
• Journal Information: J. Dairy Sci.; (United States), Vol. 66:12
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA
CHEMICAL COMPOSITION
GENE MUTATIONS
BIOLOGICAL INDICATORS
SPERMATOZOA
CYTOLOGY
MORPHOLOGY
BIOASSAY
CYTOLOGICAL TECHNIQUES
MALFORMATIONS
MAMMALS
SENSITIVITY
ANIMALS
BIOLOGY
GAMETES
GERM CELLS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
VERTEBRATES
550300* - Cytology