Developmental biochemistry of cottonseed embryogenesis and germination. XIII. Regulation of biosynthesis of principal storage proteins
The synthesis of the principal cottonseed storage proteins during embryogenesis was followed by analyses of protein synthesized in vivo and from purified RNA in vitro in the wheat germ system. The kinetics of in vivo labeling as well as immunochemical cross-reactivity indicate that the 52- and 48-kilodalton mature storage protein sets are derived from 70- and 67-kilodalton precursor protein sets that are abundant proteins in embryonic cotyledons and disappear in late embryogenesis. Identification of the initial translation products of the storage protein mRNA has not been clearly established although products of apparent molecular weights of 69,000 and 60,000 are the likely storage protein precursors. Storage protein synthesis falls off markedly in late embryogenesis simultaneously with the loss of a superabundant class of mRNAs that are presumed to be those for the storage proteins. The synthesis of these proteins ceases abruptly when immature embryos are removed from the boll and allowed to germinate precociously or when this precocious germination is prevented by incubation in abscisic acid. Thus, abscisic acid is not implicated in the expression of the storage protein genes. A scheme involving co-translational processing into vesicles, glycosylation, and slow in situ cleavage to produce the mature storage proteins is proposed.
- Research Organization:
- Univ. of Georgia, Athens, GA (United States)
- OSTI ID:
- 6151216
- Journal Information:
- Plant Physiol.; (United States), Vol. 68:1
- Country of Publication:
- United States
- Language:
- English
Similar Records
Properties and regulation of biosynthesis of cottonseed storage proteins. Comprehensive progress report, December 1, 1976 to September 1, 1979
Developmental biochemistry of cottonseed embryogenesis and germination. XII. Purification and properties of principal storage proteins
Related Subjects
60 APPLIED LIFE SCIENCES
COTTON PLANTS
ONTOGENESIS
BIOCHEMISTRY
PROTEINS
BIOSYNTHESIS
IMMUNOLOGY
MESSENGER-RNA
MOLECULAR WEIGHT
PRECURSOR
SEEDS
TIME DEPENDENCE
CHEMISTRY
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLANTS
RNA
SYNTHESIS
551000* - Physiological Systems
550200 - Biochemistry
553000 - Agriculture & Food Technology