Properties and regulation of biosynthesis of cottonseed storage proteins. Comprehensive progress report, December 1, 1976 to September 1, 1979

Technical Report · Mon Jan 01 04:00:00 EST 1979

DOI:https://doi.org/10.2172/5614038· OSTI ID:5614038

The regulation of gene expression in cotton seed embryogenesis was studied by attempting to define what gene products are likely to be highly regulated during this developmental progression. The flow of nitrogen into the free amino acids pools of the developing cotyledons, and into the principal nitrogen nutritional reserve of the seed, the storage proteins was measured. This was continued by following the flow of nitrogen from the storage proteins to the principal exported amino acid asparagine that occurs during the first several days of germination. In this fashion the rise and fall of certain enzymes of amino acid intermediary metabolism could be postulated, and in some cases, verified. The subsets of abundant mRNAs whose appearance and disappearance coincided with developmental events in cotyledon embryogenesis/germination with the short range goal of identifying proteins/enzyme activities were delineated as well as their mRNAs that represent specific developmental stages and the long range goal of using these representatives as probes for studying the mechanisms controlling the rise and fall of these mRNAs and their protein products.

• Research Organization: Georgia Univ., Athens (USA). Dept. of Biochemistry
• Sponsoring Organization: USDOE
• OSTI ID: 5614038
• Report Number(s): DOE/EV/00638-13
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMIDES
AMINO ACIDS
ASPARAGINE
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBOXYLIC ACIDS
CELL CYCLE
COTTON
CRYOGENIC FLUIDS
ELEMENTS
ENZYMES
FLUIDS
GENES
GENETIC CONTROL
GERMINATION
MESSENGER-RNA
NITROGEN
NONMETALS
NUCLEIC ACIDS
ONTOGENESIS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEST CONTROL
PROTEINS
RNA
SYNTHESIS