Studies on the interaction between the Ehrlich ascites tumor cell and its fluid environment
In this dissertation, the glycolytic nature of the Ehrlich ascites tumor (EAT) cell is disclosed both in vivo and in vitro by experiments challenging it with glucose. It is demonstrated that EAT cells can cause the extracellular pH to drop to values sufficiently acidic so as to inhibit EAT glycolysis. However, the extracellular fluid or the Ascites Supernatant Fluid (ASF) reduced the extent to which the pH dropped during EAT cell glycolysis. A comparison of the activities of the sera from tumor-bearing mice and normal mice revealed that the serumfrom the tumor-bearing mice reduced the pH fall generated by the EAT cell in the same way as did ASF; normal mouse serum had no such effect. The metabolic pathways utilized during glucose catabolism were examined by radio-respirometry and the results demonstrated that the high percentage of the glucose conversion to lactate occurred because of partial blockade of the TCA cycle. The databolism of glutamine, glutamic acid, asparagine, aspartic acid, and alanine was enhanced by ASF as determined by measuring /sup 14/CO/sub 2/ from /sup 14/C-labelled amino acids, with glutamine catabolism enhanced about three-fold. Fractionation experiments revealed that ASF contained a factor(s) responsible for this enhancement that had a molecular weight greater than 300,000 daltons and was heat-labile.
- Research Organization:
- State Univ. of New York, Stony Brook (USA)
- OSTI ID:
- 6105051
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AMINO ACIDS
METABOLISM
ASCITES TUMOR CELLS
GLYCOLYSIS
CARBON 14 COMPOUNDS
BLOOD SERUM
CARBON DIOXIDE
CULTURE MEDIA
GLUCOSE
MICE
PH VALUE
ALDEHYDES
ANIMAL CELLS
ANIMALS
CARBOHYDRATES
CARBON COMPOUNDS
CARBON OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
HEXOSES
LABELLED COMPOUNDS
MAMMALS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
RODENTS
SACCHARIDES
TUMOR CELLS
VERTEBRATES
550301* - Cytology- Tracer Techniques
550201 - Biochemistry- Tracer Techniques