Changes in Ehrlich ascites carcinoma after death of host mice. Changes of radiosensitivity of tumor cells

Journal Article · Tue Jun 20 04:00:00 EDT 1972 · Nippon Gan Chiryo Gakkai-Shi, v. 7, pp. 104-115

OSTI ID:4345180

Miura, T

A series of experimental studies were carried out using Ehrlich ascites carcinoma. The radiosensitivity of those cells under such conditions were also studied. These results throw light on the problem of how the radiosensitivity of tumor cells is affected by the changes of the surrounding condition. Following death of the host, tumor cells are placed under anerobic conditions, but glycolysis is also inhibited. In the synthetic processes of nucleic acid, DNA synthesis was markedly inhibited shortly after death of the host, whereas RNA synthesis was maintained for a considerably prolonged period of time. In other words, it was suggested that the cessation of proliferation occurs within a short period of time, but the cellular function is maintained for a certain period of time. Furthermore, DNA synthesis which appeared to have been suspended after the death revived following the transplantation of cells to living mice. Within 6 hours of death, the uptake of trypan blue in tumor cells increased and at the same time DNase activity became remarkable. Six hours after the death of the host, the transplantability of tumor cells changed also. The oxygen tension in ascites in the group of dead mice showed significant decrease as compared with the control group (living mice), together with a significant decline in the body temperature. The PH of ascites of dead mice was lower than that of the control. The ascites was drawn from the dead mice irradiated in a dosage of 500 R and 1000 R 1, 2, and 3 hours after death, and from the irradiated living mice (control). These two kinds of ascites were transplanted intraperitoneally in one group (ascites form) and subcutaneously in another group (solid form) respectively. The survival of animals of the group transplanted with ascites from ihe dead mice was shortened as compared with the control. The weight of the solid tumor deriving from dead mice was also heavier than that of the control. From these experimental results, it may be possible to conclude that the irradiation on Ehrlich ascites carcinoma is less effective in the dead than in the living host. (auth)

• Research Organization: Kobe Univ. School of Medicine, Japan
• NSA Number: NSA-29-027086
• OSTI ID: 4345180
• Journal Information: Nippon Gan Chiryo Gakkai-Shi, v. 7, pp. 104-115, Journal Name: Nippon Gan Chiryo Gakkai-Shi, v. 7, pp. 104-115; ISSN NGCJA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

*ASCITES TUMOR CELLS-- RADIOSENSITIVITY
*EHRLICH ASCITES TUMOR-- BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
DEATH
DNA
DNA-ASE
MICE
N48520* --Life Sciences--Radiation Effects on Animals-- Vertebrates
OXYGEN
PH VALUE
RADIATION DOSES
RNA
SURVIVAL TIME
TRANSPLANTS
TRYPAN BLUE