Flow cytometric evaluation of cell-cycle progression in ethyl methanesulfonate and methyl methanesulfonate-exposed P3 cells: Relationship to the induction of sister-chromatid exchanges and cellular toxicity
- National Center for Toxicological Research, Jefferson, AR (United States)
In order to determine the relationships among the reduction in relative cloning efficiency (RCE), sister-chromatid exchange (SCE) formation, and interference with progression through the cell-cycle, human teratocarcinoma-derived (P3) cells were exposed to either ethyl methanesulfonate or to methyl methanesulfonate. The relationship between SCE and toxicity was quantified, the progression through the cell-cycle was evaluated with flow cytometric methods, and the effects of these chemicals on cell growth and average generation time (AGT) were determined. A strong correlation existed between RCE and SCE which was accompanied by an inhibition of growth as evidenced by a significant negative linear effect of concentration on the he relative cell count from 24 to 72 hours after exposure and by a concentration-dependent increase. One interpretation of these results is that those effects of EMS and MMS exposure which result in S-phase delay and G{sub 2} arrest may be those elements common to the induction of SCE and cellular toxicity.
- OSTI ID:
- 6097538
- Journal Information:
- Environmental and Molecular Mutagenesis; (United States), Vol. 18:2; ISSN 0893-6692
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL CYCLE
KINETICS
EMS
GENETIC EFFECTS
METHYL METHANESULFONATE
CELL FLOW SYSTEMS
SISTER CHROMATID EXCHANGES
TOXICITY
BIOLOGICAL EFFECTS
CHROMOSOMAL ABERRATIONS
ESTERS
MUTAGENS
MUTATIONS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
SULFONIC ACID ESTERS
560300* - Chemicals Metabolism & Toxicology