Biodegradation of ddt (1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane) by the white rot fungus phanerochaete chrysosporium
Extensive biodegradation of 1,1,1-trichloro-2,2bis(4-chlorophenyl)ethane (DDT) by the white rot fungus Phanerochaete chrysosporium was demonstrated by disappearance and mineralization of (14C) DDT in nutrient nitrogen-deficient cultures. Mass balance studies demonstrated the formation of polar and water-soluble metabolites during degradation. Hexane-extractable metabolites identified by gas chromatography-mass spectrometry included 1,1-dichloro-2,2-bis(4-chlorophenyl)ethane(DDD), 2,2,2-trichloro-1,1-bis(4-chlorophenyl)ethanol (dicofol), 2,2-dichloro-1,1-bis(4-chlorophenyl) ethanol (FW-152), and 4,4'-dichlorobenzophenone (DBP). DDD was the first metabolite observed; it appeared after 3 days of incubation and disappeared from culture upon continued incubation. This, as well as the fact that ((14)C) dicofol was mineralized, demonstrates that intermediates formed during DDT degradation are also metabolized. These results demonstrate that the pathway for DDT degradation in P. chrysosporium is clearly different from the major pathway proposed for microbial or environmental degradation of DDT. Like P. chrysosporium ME-446 and BKM-F-1767, the white rot fungi Pleurotus ostreatus, Phellinus weirii, and Polyporus versicolor also mineralized DDT.
- Research Organization:
- Michigan State Univ., East Lansing, MI (United States). Dept. of Biochemistry
- OSTI ID:
- 6081213
- Report Number(s):
- PB-93-191617/XAB
- Resource Relation:
- Other Information: Pub. in Applied and Environmental Microbiology, Vol. 53, No. 9, 2001-2008(Sep 1987). See also PB--93-191575 and PB--93-191567
- Country of Publication:
- United States
- Language:
- English
Similar Records
Determination of chlorinated pesticides in urine by molecular neutron activation analysis
Polychlorinated biphenyls, 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (p,p{prime}-DDT) and 1,1-dichloro-2,2-bis(p-chlorophenyl)-ethylene (p,p{prime}-DDE) in human plasma related to fish consumption
Related Subjects
54 ENVIRONMENTAL SCIENCES
ETHANE
BIODEGRADATION
CARBON 14
CULTURE MEDIA
DBP
DDT
ETHANOL
FUNGI
GAS CHROMATOGRAPHY
HEXANE
HIGH PRESSURE
INCUBATION
INSECTICIDES
MASS BALANCE
METABOLISM
METABOLITES
MINERALIZATION
NITROGEN
PHANEROCHAETE
POLYPORUS VERSICOLOR
SPECTROMETERS
USA
ALCOHOLS
ALKANES
AROMATICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BUTYL PHOSPHATES
CARBON ISOTOPES
CHEMICAL REACTIONS
CHROMATOGRAPHY
DECOMPOSITION
DEVELOPED COUNTRIES
ELEMENTS
ESTERS
EUMYCOTA
EVEN-EVEN NUCLEI
HYDROCARBONS
HYDROXY COMPOUNDS
ISOTOPES
LIGHT NUCLEI
MEASURING INSTRUMENTS
NONMETALS
NORTH AMERICA
NUCLEI
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PESTICIDES
PHOSPHORIC ACID ESTERS
PLANTS
RADIOISOTOPES
SEPARATION PROCESSES
YEARS LIVING RADIOISOTOPES
550700* - Microbiology
540220 - Environment
Terrestrial- Chemicals Monitoring & Transport- (1990-)