Increased vitamin E content in the lung after ozone exposure: A possible mobilization in response to oxidative stress

Journal Article · Thu Nov 01 00:00:00 EST 1990 · Archives of Biochemistry and Biophysics; (USA)

DOI:https://doi.org/10.1016/0003-9861(90)90115-F· OSTI ID:6064842

Elsayed, N M; Mustafa, M G; Mead, J F ^[1]

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1. Letterman Army Institute of Research, Presidio of San Francisco, CA (USA)

Vitamin E (vE) is a biological free radical scavenger capable of providing antioxidant protection depending upon its tissue content. In previous studies, we observed that vE increased significantly in rat lungs after oxidant exposure, and we postulated that vE may be mobilized to the lung from other body sites under oxidative stress. To test this hypothesis, we fed Long-Evans rats either a vE-supplemented or a vE-deficient diet, injected them intraperitoneally with 14C-labeled vE, and then exposed half of each group to 0.5 ppm ozone (O3) for 5 days. After exposure, we determined vE content and label retention in lungs, liver, kidney, heart, brain, plasma, and white adipose tissue. Tissue vE content of all tissues generally reflected the dietary level, but labeled vE retention in all tissues was inversely related to tissue content, possibly reflecting a saturation of existing vE receptor sites in supplemented rats. Following O3 exposure, lung vE content increased significantly in supplemented rats and decreased in deficient rats, but the decrease was not statistically significant, and vE content remained unchanged in all other tissues of both dietary groups. Retention of 14C-labeled vE increased in all tissues of O3-exposed rats of both dietary groups, except in vE-deficient adipose tissue and vE-supplemented brain, where it decreased, and plasma, where it did not change. The marked increases in lung vE content and labeled vE retention of O3-exposed vE-supplemented rats support our hypothesis that vE may be mobilized to the lung in response to oxidative stress, providing that the vitamin is sufficiently available in other body sites.

• OSTI ID: 6064842
• Journal Information: Archives of Biochemistry and Biophysics; (USA), Vol. 282:2; ISSN 0003-9861
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
OZONE
TOXICITY
VITAMIN E
RETENTION
CARBON 14 COMPOUNDS
LUNGS
METABOLISM
RADICALS
RATS
RECEPTORS
TISSUE DISTRIBUTION
TRACER TECHNIQUES
ANIMALS
BODY
DISTRIBUTION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RESPIRATORY SYSTEM
RODENTS
VERTEBRATES
VITAMINS
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques