Gene mutation, quantitative mutagenesis, and mutagen screening in mammalian cells: study with the CHO/HGPRT system

Conference · Tue Jan 01 00:00:00 EST 1980

OSTI ID:6060657

Hsie, A W

We have employed CHO cells to develop and define a set of stringent conditions for studying mutation induction to TG resistance. Several lines of evidence support the CHO/HGPRT system as a specific-locus mutational assay. The system permits quantification of mutation at the HGPRT locus induced by various physical and chemical mutagens. The quantitative nature of the system provides a basis for the study of structure-function relationships of various classes of chemical mutagens. The intra- and interlaboratory reproducibility of this system suggests its potential for screening environmental agents for mutagenic activity.

• Research Organization: Oak Ridge National Lab., TN (USA)
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6060657
• Report Number(s): CONF-8010221-1; ON: DE82001496; TRN: 82-001038
• Resource Relation: Conference: NATO advanced study course, Erice, Italy, 18 Oct 1980
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
GENE MUTATIONS
GENETIC RADIATION EFFECTS
CELL CULTURES
MUTAGEN SCREENING
PERFORMANCE TESTING
MUTAGENESIS
BIOLOGICAL INDICATORS
HAMSTERS
MUTAGENS
ULTRAVIOLET RADIATION
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
MAMMALS
MUTATIONS
RADIATION EFFECTS
RADIATIONS
RODENTS
SCREENING
TESTING
VERTEBRATES
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