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Title: Gene mutation, quantitative mutagenesis, and mutagen screening in mammalian cells: study with the CHO/HGPRT system

We have employed CHO cells to develop and define a set of stringent conditions for studying mutation induction to TG resistance. Several lines of evidence support the CHO/HGPRT system as a specific-locus mutational assay. The system permits quantification of mutation at the HGPRT locus induced by various physical and chemical mutagens. The quantitative nature of the system provides a basis for the study of structure-function relationships of various classes of chemical mutagens. The intra- and interlaboratory reproducibility of this system suggests its potential for screening environmental agents for mutagenic activity.
Authors:
Publication Date:
OSTI Identifier:
6060657
Report Number(s):
CONF-8010221-1
ON: DE82001496; TRN: 82-001038
DOE Contract Number:
W-7405-ENG-26
Resource Type:
Conference
Resource Relation:
Conference: NATO advanced study course, Erice, Italy, 18 Oct 1980
Research Org:
Oak Ridge National Lab., TN (USA)
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; ANIMAL CELLS; GENE MUTATIONS; GENETIC RADIATION EFFECTS; CELL CULTURES; MUTAGEN SCREENING; PERFORMANCE TESTING; MUTAGENESIS; BIOLOGICAL INDICATORS; HAMSTERS; MUTAGENS; ULTRAVIOLET RADIATION; ANIMALS; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; ELECTROMAGNETIC RADIATION; GENETIC EFFECTS; MAMMALS; MUTATIONS; RADIATION EFFECTS; RADIATIONS; RODENTS; SCREENING; TESTING; VERTEBRATES 560121* -- Radiation Effects on Cells-- External Source-- (-1987); 560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987); 550400 -- Genetics