Calcium in the developing Ambystoma neural axis shown by 3H and fluorescent chlortetracycline and atomic absorption spectrometry
- State Univ. of New York, New Paltz (USA)
The calcium ion has been implicated in the mediation of the morphogenetic movements that occur during neural tube formation. The present study identifies high levels of calcium in the neuroepithelium of the neural plate, folds, and tube. These levels are substantially higher than those discerned elsewhere in the embryo. The calcium is localized in morphogenetically active regions by using the antibiotic chlortetracycline (CTC) which chelates calcium and is demonstrated in this investigation by both autoradiography and calcium-linked fluorescence. The specificity of CTC reaction for calcium in the developing neural axis is confirmed by EGTA competition. A comparison of the actual calcium levels in the developing neural axis (dorsal) with equivalently weighted ventral tissues was obtained by atomic absorption spectrometry (AAS). This method provides a total count of the calcium without any loss during tissue processing. For AAS, living tissues were precisely excised and immediately dessicated. Each tissue sample (dry weight 1.5 mg) was then solubilized for analysis. The spectrometric data reveal that the embryonic dorsal aspect forming the neural tube contains 57% more calcium than an equivalent weight of the ventral aspect.
- OSTI ID:
- 6057212
- Journal Information:
- Anatomical Record; (USA), Vol. 228:4; ISSN 0003-276X
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CALCIUM COMPOUNDS
BIOSYNTHESIS
SALAMANDERS
ONTOGENESIS
ABSORPTION SPECTRA
EMBRYOS
EPITHELIUM
FLUORESCENCE
NERVE CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALKALINE EARTH METAL COMPOUNDS
AMPHIBIANS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AQUATIC ORGANISMS
BODY
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LUMINESCENCE
SOMATIC CELLS
SPECTRA
SYNTHESIS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques