Development of the preoptic area: time and site of origin, migratory routes, and settling patterns of its neurons

Journal Article · Sun Nov 01 04:00:00 EST 1987 · J. Comp. Neurol.; (United States)

DOI:https://doi.org/10.1002/cne.902650106· OSTI ID:5601218

Bayer, S A; Altman, J

Neurogenesis and morphogenesis in the rat preoptic area were examined with (/sup 3/H)thymidine autoradiography. For neurogenesis, the experimental animals were the offspring of pregnant females given an injection of (/sup 3/H)thymidine on two consecutive gestational days. Nine groups were exposed to (/sup 3/H)thymidine on embryonic days E13-E14, E14-E15, E21-E22, respectively. On postnatal day P5, the percentage of labeled cells and the proportion of cells originating during 24-hr periods were quantified at four anteroposterior levels in the preoptic area. Throughout most of the preoptic area there is a lateral to medial neurogenetic gradient. Neurons originate between E12-E15 in the lateral preoptic area, between E13-E16 in the medial preoptic area, between E14-E17 in the medial preoptic nucleus, and between E15-E18 in the periventricular nucleus. These structures also have intrinsic dorsal to ventral neurogenetic gradients. There are two atypical structures: (1) the sexually dimorphic nucleus originates exceptionally late (E15-E19) and is located more lateral to the ventricle than older neurons; (2) in the median preoptic nucleus, where older neurons (E13-E14) are located closer to the third ventricle than younger neurons (E14-E17). For an autoradiographic study of morphogenesis, pregnant females were given a single injection of (/sup 3/H)thymidine during gestation, and their embryos were removed either two hrs later (short survival) or in successive 24-hr periods (sequential survival). Short-survival autoradiography was used to locate the putative neuroepithelial sources of preoptic nuclei, and sequential survival autoradiography was used to trace the migratory waves of young neurons and their final settling locations. The preoptic neuroepithelium is located anterior to and in the front wall of the optic recess.

• Research Organization: Indiana-Purdue Univ., Indianapolis
• OSTI ID: 5601218
• Journal Information: J. Comp. Neurol.; (United States), Journal Name: J. Comp. Neurol.; (United States) Vol. 265:1; ISSN JCNEA
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
AZINES
BODY
CELL CONSTITUENTS
CELL DIFFERENTIATION
CELL NUCLEI
EPITHELIUM
HETEROCYCLIC COMPOUNDS
LABELLED COMPOUNDS
MAMMALS
MOLECULAR STRUCTURE
NERVE CELLS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PREGNANCY
PROGENY
PYRIMIDINES
RATS
RIBOSIDES
RODENTS
SOMATIC CELLS
THYMIDINE
TISSUES
TRITIUM COMPOUNDS
VERTEBRATES