Simple and rapid solid-phase radioimmunoassay for serum progesterone, using the protein A of Staphylococcus aureus as immunoadsorbent

Journal Article · Wed Jul 01 00:00:00 EDT 1981 · J. Lab. Clin. Med.; (United States)

OSTI ID:6036900

Jungers, J; Delogne-Desnoeck, J; Robyn, C

A simple, rapid, and inexpensive radioimmunoassay method for serum progesterone is described, which uses a solid-phase technique for separation of antibody-bound from antibody-free progesterone. Rabbit antiprogesterone immunoglobulins are adsorbed on the protein A of formaldehyde- and heat-treated Staphylococcus aureus cells (Pansorbin; Calbiochem-Behring Corp., La Jolla, California). The suspension of antibody-coated Pansorbin retains all its binding activity of 1-2-H(N)-progesterone when kept at + 4/sup 0/ or at -25/sup 0/C for at least 4 months. Dose-response curves obtained with ether-serum extracts and with the progesterone standard do not deviate significantly from parallelism. The progesterone standard gives identical dose-response curves whether diluted in the assay buffer or in a progesterone-free ether-serum extract. The sensitivity of the assay is 0.02 ng/assay tube. The intra-assay variation coefficient is 16%, and the routine interassay variation coefficient is 17%. The mean serum progesterone concentrations were 0.55 ng/ml during the follicular phase of the menstrual cycle and 12.5 ng/ml during the luteal phase. The average blank value for distilled water was 0.02 ng/assay tube.

• Research Organization: Universite Libre de Bruxelles, Belgium
• OSTI ID: 6036900
• Journal Information: J. Lab. Clin. Med.; (United States), Vol. 98:1
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
PROGESTERONE
RADIOIMMUNOASSAY
DOSE-RESPONSE RELATIONSHIPS
EXPERIMENTAL DATA
IMMUNOGLOBULINS
PROTEINS
RABBITS
STAPHYLOCOCCUS
SUSPENSIONS
ANIMALS
BACTERIA
DATA
DISPERSIONS
GLOBULINS
HORMONES
INFORMATION
ISOTOPE APPLICATIONS
KETONES
MAMMALS
MICROORGANISMS
NUMERICAL DATA
ORGANIC COMPOUNDS
PREGNANES
RADIOASSAY
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics