Luteinizing hormone-releasing hormone levels in human plasma: a radioimmunoassay method. [/sup 125/I]
Journal Article
·
· Am. J. Obstet. Gynecol.; (United States)
A sensitive double-antibody radioimmunoassay, developed to measure luteinizing hormone-releasing hormone (LHRH) levels in fetal brain tissues, has been modified to quantitate LHRH in unextracted plasma samples. Pure, synthetic LHRH was iodinated with /sup 125/I for tracer and was also used as standard. The antibody utilized was specific for LHRH and did not cross react with other hypothalamic or pituitary hormones. The separation of free from antibody-bound tracer was performed by dextran-coated charcoal. The sensitivity of the assay was 2.5 pg/tube. The intra-assay and interassay coefficients of variation were 5.9 and 6.1% for 50 pg/ml and 11.8 and 14.9% for 25 pg/ml samples. Recovery of known amounts of LHRH added to plasma samples that had no detectable hormone was 96.4 +- 8.8%. Whether these samples were previously prepared and kept frozen at -20/sup 0/C or freshly prepared, the recovery of LHRH was consistent and quantitatively stable in each assay. Serial determinations of LHRH in plasma obtained from ovulatory volunteers averaged between 20.5 +- 2.1 pg/ml in the follicular phase and 19.4 +- 4.5 pg/ml in the luteal phase. At midcycle, coincident with the luteinizing hormone surge, LHRH levels averaged 17.6 +- 4.4 pg/ml.
- Research Organization:
- Univ. of South Alabama, Mobile
- OSTI ID:
- 6137494
- Journal Information:
- Am. J. Obstet. Gynecol.; (United States), Journal Name: Am. J. Obstet. Gynecol.; (United States) Vol. 134:6; ISSN AJOGA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIGEN-ANTIBODY REACTIONS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
DATA
DATA FORMS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ESTROUS CYCLE
EXPERIMENTAL DATA
FEMALES
HORMONES
IMMUNE SERUMS
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOLATED VALUES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LH-RH
MAMMALS
MAN
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
PATIENTS
PEPTIDE HORMONES
PITUITARY HORMONES
PRIMATES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES
VERTEBRATES
WOMEN
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIGEN-ANTIBODY REACTIONS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
DATA
DATA FORMS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ESTROUS CYCLE
EXPERIMENTAL DATA
FEMALES
HORMONES
IMMUNE SERUMS
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOLATED VALUES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LH-RH
MAMMALS
MAN
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
PATIENTS
PEPTIDE HORMONES
PITUITARY HORMONES
PRIMATES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES
VERTEBRATES
WOMEN