Marked differences in immunocytological localization of ( sup 3 H)estradiol-binding protein in rat pancreatic acinar tumor cells compared to normal acinar cells

Journal Article · Fri Mar 01 00:00:00 EST 1991 · Endocrinology; (USA)

DOI:https://doi.org/10.1210/endo-128-3-1617· OSTI ID:5922282

Beaudoin, A R; Grondin, G; St Jean, P; Pettengill, O; Longnecker, D S; Grossman, A ^[1]

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1. Universite Sherbrooke, Quebec (Canada)

({sup 3}H)Estradiol can bind to a specific protein in normal rat pancreatic acinar cells. Electron microscopic immunocytochemical analysis has shown this protein to be localized primarily in the rough endoplasmic reticulum and mitochondria. Rat exocrine pancreatic tumor cell lines, whether grown in tissue culture (AR42J) or as a tumor mass after sc injection into rats (DSL-2), lacked detectable amounts of this ({sup 3}H)estradiol-binding protein (EBP), as determined by the dextran-coated charcoal assay. Furthermore, primary exocrine pancreatic neoplasms induced with the carcinogen azaserine contained little or no detectable ({sup 3}H)estradiol-binding activity. However, electron immunocytochemical studies of transformed cells indicated the presence of material that cross-reacted with antibodies prepared against the ({sup 3}H)EBP. The immunopositive reaction in transformed cells was localized almost exclusively in lipid granules. Such lipid organelles in normal acinar cells, although present less frequently than in transformed cells, have never been observed to contain EBP-like immunopositive material. Presumably, the aberrant localization of EBP in these acinar tumor cells results in loss of function of this protein, which in normal pancreatic acinar cells appears to exert a modulating influence on zymogen granule formation and the process of secretion.

• OSTI ID: 5922282
• Journal Information: Endocrinology; (USA), Vol. 128:3; ISSN 0013-7227
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ESTRADIOL
BIOCHEMICAL REACTION KINETICS
PANCREAS
NEOPLASMS
PROTEINS
BIOLOGICAL LOCALIZATION
ELECTRON MICROSCOPY
ENDOPLASMIC RETICULUM
MITOCHONDRIA
RECEPTORS
TISSUE CULTURES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
ANIMAL CELLS
BODY
CELL CONSTITUENTS
DIGESTIVE SYSTEM
DISEASES
ENDOCRINE GLANDS
ESTRANES
ESTROGENS
GLANDS
HORMONES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
MEMBRANE PROTEINS
MICROSCOPY
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
REACTION KINETICS
STEROID HORMONES
STEROIDS
550201* - Biochemistry- Tracer Techniques
550901 - Pathology- Tracer Techniques