Lung edema due to hydrogen peroxide is independent of cyclooxygenase products

Journal Article · Sun Jan 01 00:00:00 EST 1984 · J. Appl. Physiol.: Respir., Environ. Exercise Physiol.; (United States)

OSTI ID:5862794

Burghuber, O; Mathias, M M; McMurtry, I F; Reeves, J T; Voelkel, N F

Active oxygen species can cause lung injury. Although a direct action on endothelial cells is proposed, the possibility exists that they might cause injury via mediators. We considered that active oxygen species would stimulate the generation of cyclooxygenase metabolites, which then alter pulmonary vasoreactivity and cause edema. We chemically produced hydrogen peroxide by adding glucose oxidase to a plasma- and cell-free, but ..beta..-D-glucose-containing, solution, which perfused isolated rat lungs. Addition of glucose oxidase to the perfusate caused a marked decrease in pulmonary vasoreactivity, accompanied by an increase in the concentrations of prostacyclin, thromboxane A/sub 2/, and prostaglandin F/sub 2..cap alpha../. Pretreatment with catalase, a specific scavenger of hydrogen peroxide, preserved pulomonary vasoreactivity, inhibited the increase of the concentration of the measured prostaglandins, and prevented edema formation. Indomethacin effectively blocked lung prostaglandin production but neither prevented the decrease in vasoreactivity nor inhibited edema formation. From these data we conclude the hydrogen peroxide impaired pulmonary vasoreactivity and subsequently caused edema. Depsite the fact that hydrogen peroxide stimulated lung prostaglandin production, cyclooxygenase-derived products neither caused the decrease in vasoreactivity nor the development of edema.

• Research Organization: Univ. of Colorado Health Sciences Center, Denver
• OSTI ID: 5862794
• Journal Information: J. Appl. Physiol.: Respir., Environ. Exercise Physiol.; (United States), Vol. 56:4
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
HYDROGEN PEROXIDE
TOXICITY
LUNGS
EDEMA
OXYGENASES
ENZYME ACTIVITY
CATALASE
ENDOTHELIUM
GLUCOSE
INJURIES
METABOLITES
PERFUSED ORGANS
PROSTAGLANDINS
RATS
ALDEHYDES
ANIMAL TISSUES
ANIMALS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
ENZYMES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HEXOSES
HYDROGEN COMPOUNDS
MAMMALS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PATHOLOGICAL CHANGES
PEROXIDASES
PEROXIDES
PORPHYRINS
RESPIRATORY SYSTEM
RODENTS
SACCHARIDES
SYMPTOMS
TISSUES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550200 - Biochemistry