Solubilization of glycoproteins of envelope viruses by detergents
The action of a number of known ionic and nonionic detergents, as well as the new nonionic detergent MESK, on envelope viruses was investigated. It was shown that the nonionic detergents MESK, Triton X-100, and octyl-..beta..-D-glucopyranoside selectively solubilize the outer glycoproteins of the virus particles. The nonionic detergent MESK has the mildest action. Using MESK, purified glycoproteins of influenza, parainfluenza, Venezuelan equine encephalomyelitis, vesicular stomatitis, rabies, and herpes viruses were obtained. The procedure for obtaining glycoproteins includes incubation of the virus suspension with the detergent MESK, removal of subvirus structures by centrifuging, and purification of glycoproteins from detergents by dialysis. Isolated glycoproteins retain a native structure and biological activity and possess high immunogenicity. The detergent MESK is promising for laboratory tests and with respect to the production of subunit vaccines.
- Research Organization:
- D.I. Ivanovskii Institute of Virology, Moscow, USSR
- OSTI ID:
- 5856548
- Journal Information:
- Biochemistry (Engl. Transl.); (United States), Vol. 51:5; Other Information: Translated from Biokhimiya; 51: No. 5, 808-815(May 1986)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DETERGENTS
BIOLOGICAL EFFECTS
LABELLING
GLUCOPROTEINS
DISSOLUTION
RADIOIMMUNOASSAY
VIRUSES
BIODEGRADATION
CARBON 14 COMPOUNDS
COMPARATIVE EVALUATIONS
ELECTROPHORESIS
HEMAGGLUTININS
IMMUNE REACTIONS
MATERIALS TESTING
RADIOACTIVITY
SOLUBILITY
ADDITIVES
AGGLUTININS
ANTIBODIES
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
EMULSIFIERS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MICROORGANISMS
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RADIOASSAY
RADIOIMMUNOLOGY
SACCHARIDES
SURFACTANTS
TESTING
TRACER TECHNIQUES
WETTING AGENTS
550201* - Biochemistry- Tracer Techniques