Quantitation of vitamin B6 in biological samples by isotope dilution mass spectrometry
Methods have been developed for the simultaneous quantitative analysis of vitamin B6 forms in biological samples by isotope dilution mass spectrometry using deuterated forms of pyridoxine, pyridoxal, pyridoxamine, and pyridoxic acid. The biological fluid or tissue sample was homogenized and then treated with a cocktail containing appropriate amounts of each deuterated vitamer, as well as the deuterated, phosphorylated vitamer forms. The individual vitamers were isolated from the homogenate by a complex high-performance liquid chromatographic procedure that provided separate fractions for each of the six vitamers found in biological samples. Aldehydic B6 vitamers were reduced to the alcohol form prior to acetylation and analysis by gas chromatography/mass spectrometry (GC/MS). The three resulting vitamers were analyzed by electron ionization GC/MS using a silicone capillary column. The methods have been applied to analysis of vitamin B6 in liver, milk, urine, and feces at levels as low as 0.02 nmol/ml.
- Research Organization:
- Texas Children's Hospital, Houston
- OSTI ID:
- 5844606
- Journal Information:
- Anal. Biochem.; (United States), Vol. 1
- Country of Publication:
- United States
- Language:
- English
Similar Records
Uptake of N-(4 prime -pyridoxyl)amines and release of amines by renal cells: A model for transporter-enhanced delivery of bioactive compounds
Radiometric-microbiologic assay fo vitamin B-6: analysis of plasma samples
Related Subjects
PYRIDOXINE
ISOTOPE DILUTION
DEUTERIUM
FECES
GUINEA PIGS
LIVER
MASS SPECTROSCOPY
MILK
QUANTITATIVE CHEMICAL ANALYSIS
URINE
ANIMALS
AZINES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY
BODY FLUIDS
CHEMICAL ANALYSIS
DIGESTIVE SYSTEM
FOOD
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MATERIALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIDINES
RODENTS
SPECTROSCOPY
STABLE ISOTOPES
TRACER TECHNIQUES
VERTEBRATES
VITAMIN B GROUP
VITAMINS
WASTES
550201* - Biochemistry- Tracer Techniques