Immunochemical methods for quantitation of vitamin B6. Technical report
Technical Report
·
OSTI ID:5313012
A procedure is described which proposes schemes for determining the total of all B6 vitamins in acid-hydrolyzed samples utilizing a radio-immunoassay (RIA) or an enzyme-immunoassay (EIA). Sample preparation is similar for both RIA and EIA. Two specific antibodies (antipyridoxine and antipyridoxamine) are employed to determine pyridoxamine, a portion of the sample is reduced with sodium borohydride. Pyridoxal is determined by difference between pyridoxine before and after reduction. The results indicate that two procedures have been developed which are selective for pyridoxamine (the fluorescent enzyme immunoassay and the spin immunoassay) and one assay which is equally sensitive to pyridoxine and pyridoxamine (the radio-immunoassay).
- Research Organization:
- Army Natick Research and Development Command, MA (USA)
- OSTI ID:
- 5313012
- Report Number(s):
- AD-A-133108/1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
AZINES
CHEMICAL REACTIONS
DECOMPOSITION
EQUIPMENT
ESTERS
FOOD
HETEROCYCLIC COMPOUNDS
HYDROLYSIS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LIGANDS
LYSIS
METABOLISM
NUTRITION
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
PROTEINS
PYRIDINES
PYRIDOXINE
RADIOASSAY
RADIOIMMUNOASSAY
SAMPLERS
SOLVOLYSIS
TRACER TECHNIQUES
VITAMIN B GROUP
VITAMINS
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
AZINES
CHEMICAL REACTIONS
DECOMPOSITION
EQUIPMENT
ESTERS
FOOD
HETEROCYCLIC COMPOUNDS
HYDROLYSIS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LIGANDS
LYSIS
METABOLISM
NUTRITION
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
PROTEINS
PYRIDINES
PYRIDOXINE
RADIOASSAY
RADIOIMMUNOASSAY
SAMPLERS
SOLVOLYSIS
TRACER TECHNIQUES
VITAMIN B GROUP
VITAMINS