High interindividual restriction fragment length and copy number of polymorphism of a TVRI family in moderate human DNA repeats
Journal Article
·
· Bull. Exp. Biol. Med. (Engl. Transl.); (United States)
The authors describe the selection of cloned human DNA sequences, with a copy number not exceeding 1000 copies per diploid genome, and their testing for interindividual restriction fragment lengths and copy number of polymorphism (RFLCP). As a result of the investigation a DNA clone was found (TVRI-6), about 2.8 kilobase-pairs in size, for which an unusually high level of interindividual RFLCP was discovered. The TVRI-6 sequence was obtained from a bank of Pst I restriction fragments of human placental nuclear DNA cloned in pBR 322. The bank was analyzed by hybridization of colonies with phosphorus 32-labelled human nuclear DNA.
- Research Organization:
- All-Union Mental Health Research Center, Moscow, USSR
- OSTI ID:
- 5841798
- Journal Information:
- Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Vol. 103:1; Other Information: Translated from Byulleten' Ehksperimental'noj Biologii i Meditsiny; 103: No. 1, 57-58(Jan 1987)
- Country of Publication:
- United States
- Language:
- English
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+1 more
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
DNA
AUTORADIOGRAPHY
HYBRIDIZATION
LABELLING
MAN
DNA SEQUENCING
TRACER TECHNIQUES
COLONY FORMATION
DNA REPLICATION
DNA-CLONING
GENES
GENETICS
LABELLED COMPOUNDS
LYMPHOCYTES
NUCLEOTIDES
PHOSPHORUS 32
PLACENTA
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGY
BLOOD
BLOOD CELLS
BODY FLUIDS
CLONING
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
FETAL MEMBRANES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
MAMMALS
MATERIALS
MEMBRANES
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550401* - Genetics- Tracer Techniques
550201 - Biochemistry- Tracer Techniques
DNA
AUTORADIOGRAPHY
HYBRIDIZATION
LABELLING
MAN
DNA SEQUENCING
TRACER TECHNIQUES
COLONY FORMATION
DNA REPLICATION
DNA-CLONING
GENES
GENETICS
LABELLED COMPOUNDS
LYMPHOCYTES
NUCLEOTIDES
PHOSPHORUS 32
PLACENTA
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGY
BLOOD
BLOOD CELLS
BODY FLUIDS
CLONING
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
FETAL MEMBRANES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
MAMMALS
MATERIALS
MEMBRANES
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550401* - Genetics- Tracer Techniques
550201 - Biochemistry- Tracer Techniques