Pst I restriction fragment length polymorphism of the human placental alkaline phosphatase gene in normal placentae and tumors
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
The structure of the human placental alkaline phosphatase gene from normal term placentae was studied by restriction enzyme digestion and Southern blot analysis using a cDNA probe to the gene for the placental enzyme. The DNA digests fall into three distinct patterns based on the presence and intensity of an extra 1.1-kilobase Pst I Band. The extra 1.1-kilobase band is present in 9 of 27 placenta samples, and in 1 of these samples the extra band is present at double intensity. No polymorphism was revealed by digestion with restriction enzymes EcoRI, Sma I, BamHI, or Sac I. The extra Pst I-digestion site may lie in a noncoding region of the gene because no correlation was observed between the restriction fragment length polymorphism and the common placental alkaline phosphatase alleles identified by starch gel electrophoresis. In addition, because placental alkaline phosphatase is frequently re-expressed in neoplasms, the authors examined tissue from ovarian, testicular, and endometrial tumors and from BeWo choriocarcinoma cells in culture. The Pst I-DNA digestion patterns from these cells and tissues were identical to those seen in the normal ovary and term placentae. The consistent reproducible digestion patterns seen in DNA from normal and tumor tissue indicate that a major gene rearrangement is not the basis for the ectopic expression of placental alkaline phosphatase in neoplasia.
- Research Organization:
- Stanford Univ. School of Medicine, CA (USA)
- OSTI ID:
- 5381446
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:13; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE PHOSPHATASE
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYME IMMUNOASSAY
ENZYMES
ESTERASES
FETAL MEMBRANES
GENES
GENETICS
HYDROLASES
IMMUNOASSAY
ISOTOPES
LIGHT NUCLEI
MEMBRANES
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHATASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLACENTA
RADIOISOTOPES
RECOMBINANT DNA
TUMOR CELLS
59 BASIC BIOLOGICAL SCIENCES
ALKALINE PHOSPHATASE
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYME IMMUNOASSAY
ENZYMES
ESTERASES
FETAL MEMBRANES
GENES
GENETICS
HYDROLASES
IMMUNOASSAY
ISOTOPES
LIGHT NUCLEI
MEMBRANES
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHATASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLACENTA
RADIOISOTOPES
RECOMBINANT DNA
TUMOR CELLS